Takara IVTpro¢â ½Ã¸®ÁîÀÇ ¾÷±×·¡ÀÌµå ½ÅÁ¦Ç° Ãâ½Ã!!
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Takara IVTpro¢â mRNA Synthesis System (Code 6141)Àº °í¼öÀ² mRNA ÇÕ¼ºÀ» À§ÇÑ all-in-one kit·Î mRNA ÇÕ¼ºÀ» °£´ÜÇÏ°Ô ¼¼ÆÃÇÒ ¼ö ÀÖ°í ½ÃÁß¿¡¼ °¡Àå ³ôÀº ¼öÀ² (20 §¡ ¹ÝÀÀ ´ç ÃÖ´ë 200 §¶ mRNA ÇÕ¼º)À̶ó´Â Á¡ÀÌ Æ¯Â¡ÀÔ´Ï´Ù. ÀÌ Á¦Ç°¿¡¼ T7 RNA Polymerase¿Í IVT template vector¸¦ ¾÷±×·¡À̵åÇÑ ½ÅÁ¦Ç°ÀÌ Ãâ½ÃµÆ½À´Ï´Ù.
Takara
IVTpro¢â mRNA Synthesis System (low dsRNA) (Code 6131)
- IVT ¹ÝÀÀ¿¡ PrimeCap¢â T7 RNA Polymerase (low dsRNA) (Code 2560A)À» »ç¿ëÇØ dsRNA »ý¼ºÀ» Å©°Ô ¾ïÁ¦ÇØ »ýüÅõ¿©½Ã ¸é¿ª¿ø¼º °¨¼Ò (±×¸² 1)
- Template vector·Î Template
Vector (BspQ I) for T7 mRNA SynthesisÀ»
»ç¿ëÇØ Poly(A) ¼¿ ÀÌÈÄ Ãß°¡¼¿ÀÌ ¾ø´Â scarless
mRNA ÇÕ¼º °¡´É (translation È¿À²ÀÌ ³ôÀº mRNA
ÇÕ¼º)
±×¸² 1. Fluc mRNA (1.9kb)ÀÇ ÇÕ¼º ¼öÀ² (A), dsRNA ÇÔ·® (B), ´Ü¹éÁú ¹ßÇö ¼öÁØ (C) ºñ±³.
Takara IVTpro¢â ½Ã¸®Áî´Â ¾Æ·¡¿Í °°ÀÌ ´Ù¾çÇÑ Á¶ÇÕÀ¸·Î ÆǸŵǰí ÀÖÀ¸´Ï ¸¹Àº °ü½É ºÎŹµå¸³´Ï´Ù.
* Plasmid Á¦ÀÛ ÈÄ linearization½Ã BspQ I (Code 1227A) »ç¿ë
** Plasmid Á¦ÀÛ ÈÄ linearization½Ã Hind III (Code 1060A) »ç¿ë
Á¦Ç° °ü·Ã
¹®ÀÇ´Â °í°´Áö¿ø(02-2081-2510, [email protected])À¸·Î ¿¬¶ô¹Ù¶ø´Ï´Ù.
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