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Home > ÀüÁ¦Ç°º¸±â > Gene transfer (virus) > Adenovirus > Adenovirus Á¤Á¦ (Maxi type)

Adenovirus Á¤Á¦ (Maxi type)

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Clontech
631533
Adeno-X Maxi Purification Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
6 ȸ
(Package)
1,673,600¿ø 
2,092,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x32392, x32393

  • Maxi prepÀ» ÅëÇÏ¿© large scale·Î ¹è¾çµÈ ÀçÁ¶ÇÕ adenovirus Á¤Á¦ °¡´É
  • Maxi kitÀº 5x15 cm plate Á¤Á¦ °¡´É
  • CsClÀ̳ª ÃÊ°í¼Ó¿ø½ÉºÐ¸®±â°¡ ºÒÇÊ¿äÇÑ chromatograpy Á¤Á¦
ClontechÀÇ chromatograpy ±â¹ÝÀÇ Adeno-X Purification Kit´Â maxi prep(5 x 15 cm plate) ±Ô¸ðÀÇ ¹è¾ç¾×À¸·ÎºÎÅÍ 1.5 ½Ã°£ À̳»¿¡ ÃÖ´ë 1013 ±îÁö Á¤Á¦ÇÒ ¼ö ÀÖ´Ù. Adeno-X Maxi Purification Kit´Â ³ÐÀº ¹ÙÀÌ·¯½º Á¤Á¦ ¿ë·®À» °¡Áö°í À־ ÃÖ´ë 1 x 1012 adenovirus ÀÔÀÚ±îÁö Á¤Á¦ ÇÒ ¼ö ÀÖ´Ù.
* Mega ±Ô¸ðÀÇ ¹è¾ç¾× (25 x 15 cm plate)À¸·ÎºÎÅÍ Adenovirus¸¦ Á¤Á¦ÇÏ´Â °æ¿ì Adeno-X Mega Purification Kit (Code 631032) Á¦Ç°À» ÃßõÇÕ´Ï´Ù.
°£´ÜÇÑ Á¤Á¦ °úÁ¤
Á¶ÀÛ °úÁ¤¿¡´Â ÃÊ°í¼Ó¿ø½ÉºÐ¸® ´Ü°è°¡ Æ÷ÇԵǾî ÀÖÁö ¾ÊÀº ´ë½Å adenovirus ÀÔÀÚ¸¸À» ¼±ÅÃÀûÀ¸·Î °áÇÕ½ÃÅ°´Â ¸Å¿ì µ¶Æ¯ÇÑ ¸·À» »ç¿ëÇÏ´Â chromatography Á¤Á¦ ¹æ¹ýÀ¸·Î adenovirus¸¦ Á¤Á¦ÇÑ´Ù. ¸·¿¡ °áÇÕµÈ ¹ÙÀÌ·¯½º ÀÔÀÚ´Â ÃÖÃÊÀÇ ¼¼Ã´ °úÁ¤À» °ÅÄ¡¸é¼­ Á¤Á¦µÈ ¹ÙÀÌ·¯½º¸¸ ¹èÃâµÇµµ·Ï ÇÑ´Ù (Figure 1). °¢ Á¦Ç°¿¡´Â Á¤Á¦¿¡ ÇÊ¿äÇÑ buffer¿Í ±× ¿Ü ¹°Ç°ÀÌ ¸ðµÎ Æ÷ÇԵǾî ÀÖ´Ù.
°íÇ°ÁúÀÇ ¹ÙÀÌ·¯½º¸¦ ³ôÀº ¿ª°¡·Î »ý»ê
ClontechÀÇ Adeno-X Purification Kit¸¦ »ç¿ëÇÏ¿© CsCl ¹Ðµµ ±¸¹è ¿ø½ÉºÐ¸®¹ý (CsCl density gradient centrifugation)º¸´Ù ¸Å¿ì ³ôÀº ¿ª°¡¿Í °í¼øµµÀÇ ¹ÙÀÌ·¯½º¸¦ ¾òÀ» ¼ö ÀÖ´Ù. CsCl¹ý°ú ´Þ¸® ClontechÀÇ ½ÇÇè¹ýÀº ¾ÈÁ¤ÀûÀ̸ç, ¸¹Àº ±â¼úÀÌ ¿äÇÏÁö ¾ÊÀ» »Ó¸¸ ¾Æ´Ï¶ó °í°¡ÀÇ Àåºñµµ ÇÊ¿äÇÏÁö ¾Ê´Ù. ¿ÀÁ÷ °£´ÜÇÑ Á¶ÀÛÀ¸·Î ³ôÀº ¼öµæÀ²ÀÇ Á¤Á¦µÈ adenovirus¸¦ ¾ÈÁ¤ÀûÀ¸·Î »ý»êÇÒ ¼ö ÀÖ´Ù.


Figure 1. Overview of the Adeno-X Maxi Purification protocol.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
References
1. Adenovirus Purification Kit (2007) Clontechniques XXII(3):6-7.

Keyword : ¹ÙÀÌ·¯½ºÁ¤Á¦,¹ÙÀÌ·¯½º Á¤Á¦,Á¤Á¦,Adenovirus Á¤Á¦,Virus Á¤Á¦,Virus purification

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