[¼±Åð¡À̵å] Immune-seq
Immune-seq ¼±Åð¡À̵å
´ÙÄ«¶ó¹ÙÀÌ¿À´Â ¸é¿ª ¼¼Æ÷ÀÇ ¹ß´Þ°ú ¸é¿ª Áúº´, ¾ÏÀÇ ¸ÞÄ¿´ÏÁòÀ» ¿¬±¸ÇÏ°í, ¸é¿ª Ä¡·áÁ¦ÀÇ °³¹ß¿¡ ±â¿©ÇÏ°íÀÚ, Àΰ£°ú »ýÁãÀÇ T cell, B cell, ±×¸®°í À̵éÀÇ receptor repertoire ºÐ¼®À» À§ÇÑ ´Ù¾çÇÑ Á¦Ç°À» Á¦°øÇÑ´Ù.
1. Targeted sequencing - TCR, BCR ProfilingÀ» À§ÇÑ repertoire ºÐ¼®
T cell ȤÀº B cellÀÇ receptor repertoire ºÐ¼®À» À§ÇÑ Á¦Ç°µéÀº ´ç»çÀÇ SMART (Switching Mechanism at 5' End of RNA Template) ±â¼úÀ» È°¿ëÇÏ¿©, 5¡¯ RACE¿Í NGS¸¦ Á¶ÇÕÇÔÀ¸·Î½á, ¹Î°¨µµ ÀÖ´Â immune repertoire sequencingÀ»
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- TCR¥á, TCR¥â chain ºÐ¼®
- BCR Heavy chain (H), Kappa chain (¥ê), Lamda chain (¥ë) °³º° ¶Ç´Â µ¿½Ã ºÐ¼®
2. Whole transcriptome analysis - Immune cellÀÇ single cell full-length ºÐ¼®