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[Overview] Mammalian Expression System

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Mammalian expression Overview

Mammalian expression system

 

Applications/advantages

CHOgro¢ç High Yield
Expression System

  • Suspension CHO cellÀ» ÀÌ¿ëÇÑ Ç×ü/´Ü¹éÁú ¹ßÇö¿ë transfection & medium system
  • Chemically defined, hydrolysate-free, animal origin-free medium Æ÷ÇÔ
  • Ç×ü, ´Ü¹éÁúÀ» °í¼öÀ²·Î ¹ßÇö
  • Dry power, polybag µî ´Ù¾çÇÑ ÇüÅÂÀÇ medium º°µµ ÆǸÅ

Human Cell-Free
Protein
Expression System

  • Á÷Á¢ ¼¼Æ÷¸¦ ¹è¾çÇÏÁö ¾Ê°í, Human cell extract¸¦ ÀÌ¿ëÇÑ in vitro ´Ü¹éÁú ÇÕ¼º ½Ã½ºÅÛ
  • ÇϳªÀÇ Æ©ºê¿¡¼­ RNA Àü»çü·ÎºÎÅÍ ´Ü¹éÁú ÇÕ¼º±îÁö ¿Ï·á
  • 150 kDaÀÌ»óÀÇ °íºÐÀÚ ´Ü¹éÁú ÇÕ¼º¿¡µµ ÀÀ¿ë °¡´É
  • His-Tag³ª c-Myc-tagged protein expression vector º°µµ ÆǸÅ

pHEK293 Ultra
Expression Vectors

  • Human HEK293 ¼¼Æ÷¿¡¼­ transient overexpression¿¡ ÃÖÀûÈ­µÈ ´Ü¹éÁú ¹ßÇö ½Ã½ºÅÛ
  • TAR-Tat systemÀ» ÀÌ¿ëÇÏ¿©, ±âÁ¸ÀÇ CMV À¯·¡ ÇÁ·Î¸ðÅÍ ½Ã½ºÅÛº¸´Ù ÃÖ´ë 10¹è ³ôÀº
    ´Ü¹éÁú ¹ßÇö °¡´É

HA-tag & Myc-tag
Expression System

  • 10 §¶/§¢ - 10 §¶/§¢
  • N- / C-¸»´ÜÀÇ HA-tagged ¶Ç´Â Myc-tagged expression vector Æ÷ÇÔ
  • HA-tagging ´Ü¹éÁú ¶Ç´Â Myc-tagging ´Ü¹éÁúÀ» ¹ßÇö½ÃÄÑ Western blot, ELISA,
    immunoprecipitation, immunostaining µî¿¡ Àû¿ë °¡´É