¸ÖƼÇà fragment cloning, ¹» °í¹ÎÇØ?
¿©·¯ °³ÀÇ insert¸¦ ÇϳªÀÇ vector¿¡ »ðÀÔÇÏ´Â Multiple fragment Cloning!
In-Fusion¢çÀ» »ç¿ëÇϸé ÃÖ´ë 5°³ insert (1 kb ±âÁØ) ¸¦ ÇÑ ¹ø¿¡ cloning ÇÒ ¼ö ÀÖ½À´Ï´Ù.
¡Ø °¢ insert´Â single band·Î
Á¤Á¦Çؼ »ç¿ëÇØ¾ß È¿°úÀûÀ¸·Î multiple cloningÀ» ÁøÇàÇÒ ¼ö ÀÖ½À´Ï´Ù.
>> In-Fusion¢ç ±â¼ú ÀÚ¼¼È÷ º¸±â
*°ü·Ã Á¦Ç°
- °¡¿ ¾øÀÌ agarose gel¿¡¼ DNA Á¤Á¦ kit :
TaKaRa MiniBEST Agarose Gel DNA Extraction Kit (Code 9762A)
- 15ºÐ ¿Ï·á! Streamlined multiple fragment cloning:
In-Fusion¢ç Snap Assembly Master Mix (Code 638947)
- In-Fusion¢çÀÇ Â¦²á Competent cells :
Stellar¢â Competent Cells (Code 636763)