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Home > ´ÙÄ«¶ó ½ºÆ©µð¿À > ¼îÄÉÀ̽º > ¸ÖƼÇà fragment cloning, ¹» °í¹ÎÇØ?


¸ÖƼÇà fragment cloning, ¹» °í¹ÎÇØ?
¿©·¯ °³ÀÇ insert¸¦ ÇϳªÀÇ vector¿¡ »ðÀÔÇÏ´Â Multiple fragment Cloning!

In-Fusion¢çÀ» »ç¿ëÇϸé ÃÖ´ë 5°³ insert (1 kb ±âÁØ) ¸¦ ÇÑ ¹ø¿¡ cloning ÇÒ ¼ö ÀÖ½À´Ï´Ù.

¡Ø °¢ insert´Â single band·Î Á¤Á¦Çؼ­ »ç¿ëÇØ¾ß È¿°úÀûÀ¸·Î multiple cloningÀ» ÁøÇàÇÒ ¼ö ÀÖ½À´Ï´Ù.

>> In-Fusion¢ç ±â¼ú ÀÚ¼¼È÷ º¸±â

*°ü·Ã Á¦Ç°
- °¡¿­ ¾øÀÌ agarose gel¿¡¼­ DNA Á¤Á¦ kit : TaKaRa MiniBEST Agarose Gel DNA Extraction Kit (Code 9762A)
- 15ºÐ ¿Ï·á! Streamlined multiple fragment cloning: In-Fusion¢ç Snap Assembly Master Mix (Code 638947)
- In-Fusion¢çÀÇ Â¦²á Competent cells : Stellar¢â Competent Cells (Code 636763)