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Home > ´ÙÄ«¶ó ½ºÆ©µð¿À > ¼îÄÉÀ̽º > TA-cloningÀº TA-kara¿¡¼­


TA-cloningÀº TA-kara¿¡¼­
Taq DNA Polymerase¿Í °°Àº PCR È¿¼Ò·Î ÁõÆøµÈ PCR »ê¹°Àº 3£§¸»´Ü¿¡ A¿°±â°¡ ºÎ°¡µÇ¾î ÀÖ¾î Æí¸®ÇÏ°Ô T-vector¿¡ ¹Ù·Î cloningÇÒ ¼ö ÀÖ½À´Ï´Ù. À̸¦ TA-cloningÀ̶ó°í Çϸç, PCR »ê¹°ÀÌ ³Ê¹« Àû°Å³ª Á¦ÇÑÈ¿¼Ò Àý´ÜÀÌ Àß ÀÌ·ç¾îÁöÁö ¾ÊÀ» ¶§ ¸Å¿ì À¯¿ëÇÏ°Ô È°¿ëµÇ°í ÀÖ½À´Ï´Ù.

´ç»ç´Â È¿À²ÀûÀÎ TA-cloningÀ» À§ÇØ Â÷º°È­µÈ °íÈ¿À² ligation premix°¡ Æ÷ÇÔµÈ Mighty TA-Cloning Kit (Code 6028)¸¦ °ø±ÞÇÏ°í ÀÖ½À´Ï´Ù.
¶ÇÇÑ blunt end PCR »ê¹°ÀÇ TA-cloningÀÇ °æ¿ì, °íÈ¿À² ligation premix¿Í A-overhang mixture°¡ Æ÷ÇÔµÈ Mighty TA-cloning Reagent Set for PrimeSTAR (Code 6019)·Î ³ôÀº ¼º°ø·ü·Î °£´ÜÇÏ°Ô TA-cloningÀÌ °¡´ÉÇÕ´Ï´Ù.

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Code

Á¦Ç°¸í

TA Cloning Kit

6028

Mighty TA-Cloning Kit

6019

Mighty TA-cloning Reagent Set for PrimeSTAR¢ç

T vector

3270

T-Vector pMD20

ÇüÁúÀüȯ¿ë Competent cells

9128

E. coli HST08 Premium Competent Cells

Colony PCR

RR310A

EmeraldAmp¢ç GT PCR Master Mix

RR350A

SapphireAmp¢ç Fast PCR Master Mix

Plasmid DNA Á¤Á¦

9760A

TaKaRa MiniBEST Plasmid Purification Kit

½Ç¿Â¿¡¼­ gelÀ» ³ì¿©
DNA ´ÜÆí ½Å¼Ó Á¤Á¦

9762A

TaKaRa MiniBEST Agarose Gel DNA Extraction Kit

Gel cutting ¾øÀÌ DNA ȸ¼ö

57064

FlashGel¢â Recovery Kit

°íÈ¿À²ÀÇ Ligation premix

6023

DNA Ligation Kit £¼Mighty Mix£¾

Á¦ÇÑÈ¿¼Ò/Ligation ¾øÀÌ
PCR cloning

638947

In-Fusion¢ç Snap Assembly Master Mix