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Capturem technology mechanism
Capturem¢âÀÌ ¾î¶»°Ô ´Ù¾çÇÑ ´Ü¹éÁú, Ç×ü, Extracellular Vesicle (EV)¸¦ Á¤Á¦ÇÏ´ÂÁö ±Ã±ÝÇϽÃÁÒ? µ¿¿µ»óÀ¸·Î ½±°Ô È®ÀÎÇϼ¼¿ä!
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Capturem technology applications
´Ù¾çÇÏ°Ô È°¿ëµÇ´Â Capturem¢â ±â¼ú! ³» »ùÇÃÀÌ ¾î¶² ¹æ½ÄÀ¸·Î Àû¿ëµÉ ¼ö ÀÖÀ»Áö ¾Ë¾Æº¸¾Æ¿ä~.
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In-Fusion Cloning mechanism
Any insert, Any vector, Any site!
In-Fusion CloningÀÌ ¾î¶»°Ô ÀÛ¿ëÇÏ´ÂÁö ±Ã±ÝÇϽÃÁÒ? SeamlessÇÑ cloningÀ» so EasyÇÏ°Ô ÇÏ´Â ¹æ¹ý! Áö±Ý ¼Ò°³ÇÕ´Ï´Ù.
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Applications using In-Fusion Cloning
In-Fusion CloningÀ» ÀÌ¿ëÇÑ ´Ù¾çÇÑ applicationÀ» ¼Ò°³ÇÕ´Ï´Ù. Subcloning¾øÀÌ multiple cloningºÎÅÍ site mutagenesis±îÁö!
Cloning Æȹæ¹ÌÀÎÀÇ ¼¼°è·Î ÃÊ´ëÇÏ°Ú½À´Ï´Ù.
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In-Fusion Cloning vs. other seamless cloning technologies
Clontech In-Fusion CloningÀº ±× ¾î¶² PCR cloningº¸´Ù ºü¸£°í Á¤È®ÇÏ°Ô CloningÀ» ÇÒ ¼ö ÀÖµµ·Ï µµ¿Íµå¸³´Ï´Ù.
Sequence error °ÆÁ¤ ¾øÀ¸´Ï ¾ó¸¶³ª ÁÁ°Ô¿ä!
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[2016 ¿Â¶óÀμ¼¹Ì³ª] In-Fusion¢ç HD Cloning: Get the right clone, every time
°ÅÀÇ ¸ðµç ½ÇÇèÀÇ ±âº»ÀÌ µÇ´Â Ŭ·Î´×, ³Ê¹« ±âº»ÀûÀÌÁö¸¸ ¾î·Á¿ì½Å°¡¿ä? Ŭ·Î´×ÀÌ µÇÁö ¾Ê¾Æ °í¹Î¸¸ ÇÏ°í ÀÖÁø ¾ÊÀº°¡¿ä?
¸ðµç °í¹ÎÀ» ³¯·Áµå¸± ȹ±âÀûÀÎ PCR Cloning ¹æ½ÄÀÇ In-Fusion¢ç HD Cloning¿¡ ´ëÇÑ ¿ø¸®¿Í Àû¿ë¹ý, ¼ö¸¹Àº ¿¬±¸ÀÚµéÀÇ »ý»ýÇÑ »ç¿ëÈı⸦ ¼Ò°³ÇÕ´Ï´Ù.
In-Fusion¢ç HD Cloning
- 15bp »óµ¿¼¿À» ÀÌ¿ëÇÑ Fusion PCR Cloning (since 2002)
- 15ºÐÀÌ¸é ¿Ï·áµÇ´Â ºü¸¥ Ŭ·Î´× ¹ÝÀÀ
- Any vector, Any insert, Any site OK
- ¼ö¸¹Àº ¿¬±¸ÀÚµéÀÌ Á÷Á¢ ÀÎÁ¤ÇÑ °íÈ¿À² Ŭ·Î´×
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[2016 ¿Â¶óÀμ¼¹Ì³ª] A SMARTer Approach to Low Input Small-RNA Sequencing
Small RNA (miRNA, siRNA, piRNA) ½ÃÄö½Ì ½Ã, »ùÇÃÀÇ ¾çÀÌ ³Ê¹« Àû¾î¼ ¶Ç´Â data representationÀÌ ÁÁÁö ¾Ê¾Æ¼ °í¹ÎÇϼ̳ª¿ä?
SMARTer¢ç smRNA-Seq Kit for Illumina¢ç´Â ÆǸŠÁßÀÎ small RNA-sequencing kit Áß, °¡Àå ÀûÀº ¾çÀÇ Total RNA (1ng) ·ÎºÎÅÍ ÀçÇö¼º°ú ¹Î°¨µµ°¡ ³ôÀº NGS ºÐ¼®ÀÌ °¡´ÉÇϵµ·Ï µµ¿Íµå¸³´Ï´Ù.
½ÇÇèÀÇ ÇѰ踦 ±Øº¹ÇÏ´Â Clontech SMART-Seq ±â¼úÀ» Àû¿ëÇÑ small RNA-SequencingÀ» º» ¼¼¹Ì³ª¿¡¼ ¸¸³ªº¸¼¼¿ä.
That¡¯s GOOD Science!
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[2016 ¿Â¶óÀμ¼¹Ì³ª] SMART Solution for single cell RNA-Seq (NGS) (Korean)
¼¼Æ÷µµ °¢°¢ ¸ðµÎ ´Ù¸¥ ¼º°Ý(Ư¡)À» °¡Áö°í ÀÖ´Ù´Â °Í ¾Ë°í °è½Å°¡¿ä? ¿ì¸®´Â Áö±Ý±îÁö ¿©·¯ °³ÀÇ ¼¼Æ÷¿¡¼ ½ÇÇèÀ» ÇÏ¿´±â ¶§¹®¿¡ °¢ ¼¼Æ÷µéÀÇ Æò±Õ°ª¸¸À» ¿¬±¸ÇØ ¿Ô½À´Ï´Ù. ´õ¿í Á¤È®ÇÑ Áúº´ÀÇ ¿¬±¸¸¦ À§Çؼ´Â °¢°¢ÀÇ ¼¼Æ÷°¡ °¡Áø Ư¼ºÀ» ¿¬±¸¸¦ ÇؾßÇÕ´Ï´Ù. ƯÈ÷ ¾Ï°ú °°ÀÌ ¼¼Æ÷ÀÇ º¯ÀÌ°¡ ÀϾ´Â °æ¿ì´Â ´õ¿í Áß¿äÇÏÁÒ. Single cell ºÐ¼®À» À§Çؼ´Â ¾ÆÁÖ ¹Î°¨ÇÑ ½ÇÇè±â¼úÀ» ÇÊ¿ä·Î ÇÕ´Ï´Ù. Single cell (1 - 1,000 cells)·ÎºÎÅÍ Next Generation sequencing (NGS)À» °¡´ÉÇÏ°Ô Çϴ Ưº°ÇÑ SMART-seq ±â¼ú! ¾î¶»°Ô »ç¿ëÀ» ÇÏ°í ÀÖ´ÂÁö »ìÆ캼±î¿ä? That's Good Science!
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[2016 ¿Â¶óÀμ¼¹Ì³ª] CRISPR/Cas9 Webinar: Tools for successful CRISPR/Cas9 Genome Editing
[Do It Yourself! CRISPR/Cas9 Genome Editing]
¼º°øÀûÀÎ CRISPR/Cas9 À¯ÀüÀÚ ÆíÁýÀ» À§ÇÑ ClontechÀÇ È¿À²ÀûÀÎ Á¦Ç°À» ¼Ò°³ÇÕ´Ï´Ù. 2015³â »çÀ̾𽺰¡ ¼±Á¤ÇÑ ¡®¿ÃÇØÀÇ Çõ½ÅÀûÀÎ ±â¼ú (Breakthrough of the Year) 10°³ Áß Çϳª·Î ²ÅÈù CRISPR/Cas9 À¯ÀüÀÚ°¡À§¡¯ ´Â »ç¶÷ ¹× µ¿½Ä¹° ¼¼Æ÷¿¡¼ ƯÁ¤ À¯ÀüÀÚÀÇ DNA¸¦ ÀνÄÇÏ¿© Àý´ÜÇÏ´Â È¿¼Ò¸¦ ÀÌ¿ëÇÑ ±â¼ú·Î½á, À¯ÀüÀÚ º¯Çü/ÆíÁýÀ» À§ÇÑ Çõ½Å ±â¼ú·Î ´ëµÎµÇ°í ÀÖ½À´Ï´Ù. º» Webinar¿¡¼´Â ¼º°øÀûÀÎ CRISPR/Cas9 Genome Editing ¿ø¸® ¹× Takara ClontechÀÇ CRISPR/Cas9 Guide-it ½Ã¸®Á ¼Ò°³ÇÕ´Ï´Ù.
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[2017 ¿Â¶óÀμ¼¹Ì³ª] Viral Gene DeliveryÀÇ ¼º°ø·üÀ» ÃÖ°í·Î Çâ»ó½ÃÅ°´Â ¹æ¹ý
¹ÙÀÌ·¯½º¸¦ ÀÌ¿ëÇÑ À¯ÀüÀÚ µµÀÔ ¹æ¹ýÀÎ "Viral Gene Delivery"´Â ³ôÀº À¯ÀüÀÚ Àü´Þ Ư¼º ´öºÐ¿¡ ¸ñÀû ´Ü¹éÁúÀÇ °ú¹ßÇö ¶Ç´Â À¯ÀüÀÚ Ä¡·á µî¿¡
°¡Àå ³Î¸® ÀÌ¿ëµÇ°í ÀÖ½À´Ï´Ù. ÇÏÁö¸¸ ¹ÙÀÌ·¯½º ÀÔÀÚÀÇ Á¦ÀÛ È¿À², ÀÔÀÚÀÇ °³¼ö µî¿¡ µû¶ó¼ À¯ÀüÀÚ µµÀÔÀÇ È¿À², ³ª¾Æ°¡ ¸ñÀû ´Ü¹éÁúÀÇ ¹ßÇö¿¡
¿µÇâÀ» ¹ÌÄ¡±âµµ ÇÕ´Ï´Ù. ±×·¸´Ù¸é, ¾î¶»°Ô Viral Gene Delivery¸¦ ¼º°øÀûÀ¸·Î ÁøÇàÇÒ ¼ö ÀÖÀ»±î¿ä?
2017³â 10¿ù 18ÀÏ ÁøÇàµÇ´Â ¿Â¶óÀÎ ¼¼¹Ì³ª¿¡¼´Â ³ôÀº titer¸¦ °¡Áö´Â ¹ÙÀÌ·¯½º »ý»ê, °íÈ¿À²ÀÇ À¯ÀüÀÚ µµÀÔ µî Viral Gene DeliveryÀÇ È¿À²À»
ÃÖ°í·Î Çâ»ó½Ãų ¼ö ÀÖ´Â ´Ù¾çÇÑ Support ToolÀÇ ÃֽŠÁ¤º¸¸¦ ¼Ò°³ÇÕ´Ï´Ù!
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[2017 ¿Â¶óÀμ¼¹Ì³ª] À¯ÀüÀÚ Ä¡·áÁ¦ ¿¬±¸¸¦ À§ÇÑ È¿À²ÀûÀÎ Viral Gene Delivery System
2017³â MIT Technology Review ¼±Á¤ 10´ë Çõ½Å±â¼ú Áß Çϳª·Î ¼±Á¤µÈ À¯ÀüÀÚ Ä¡·á 2.0 (Gene Therapy 2.0).
Ä¡¸íÀûÀÎ Èñ±Í À¯Àüº´ »Ó¸¸ ¾Æ´Ï¶ó, ¾Ï, ½ÉÀ庴, ¾ËÃ÷ÇÏÀ̸Ó, ´ç´¢ µî°ú °°Àº ÀϹÝÀûÀÎ Áúº´À» À¯ÀüÀÚ¼öÁØ¿¡¼ ±Ùº»ÀûÀ¸·Î Ä¡·áÇϱâ À§ÇÑ ´Ù¾çÇÑ À¯ÀüÀÚ Ä¡·áÁ¦°¡
¿¬±¸/°³¹ßÁß¿¡ ÀÖ½À´Ï´Ù. ½ÇÁ¦ À¯·´, ¹Ì±¹ µî¿¡¼ À¯ÀüÀÚ Ä¡·áÁ¦ÀÇ ÆǸŰ¡ ½ÂÀεǰí, ±¹³» °³¹ßÁßÀÎ À¯ÀüÀÚ Ä¡·áÁ¦ÀÇ ÇØ¿Ü ÀÓ»ó½ÂÀÎ, ±â¼úÀÌÀü µîÀÇ ¿¬±¸¼º°ú°¡ ³ª¿È¿¡ µû¶ó
ÀÌ Çõ½ÅÀûÀÎ Ä¡·á¹ý¿¡ ´ëÇÑ °ü½ÉÀÌ ³ô¾ÆÁö°í ÀÖ½À´Ï´Ù.
º» ¼¼¹Ì³ª¿¡¼´Â À¯ÀüÀÚ Ä¡·á¸¦ À§ÇÑ ´Ù¾çÇÑ delivery ¹æ¹ý Áß °¡Àå ³Î¸® »ç¿ëµÇ°í ÀÖ´Â Viral Gene Delivery System¿¡ ´ëÇØ ¾È³»µå¸®°íÀÚ ÇÕ´Ï´Ù.
¸¹Àº °ü½É°ú Âü¼®À» ºÎŹµå¸³´Ï´Ù. °¨»çÇÕ´Ï´Ù.
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[2017 ¿Â¶óÀμ¼¹Ì³ª] Single-Cell ºÐ¼®À» À§ÇÑ Çõ½ÅÀûÀÎ Á¦¾È - ICELL8 Single-Cell System.
ÃÖ±Ù Single-Cell(´ÜÀϼ¼Æ÷) ºÐ¼®ÀÌ È®´ëµÇ´Â Æ®·»µå¿¡ µû¶ó, WaferGen¿¡¼´Â ´ÜÀϼ¼Æ÷ ºÐ¼®À» À§ÇÑ ICELL8TM Single-Cell SystemÀ» °³¹ßÇÏ¿© °ø±ÞÇÏ°í ÀÖ½À´Ï´Ù.
º» ¼¼¹Ì³ª¿¡¼´Â ICELL8TM ½Ã½ºÅÛÀ» ÀÌ¿ëÇÏ¿© ¿¬±¸ÀÚ°¡ ¿øÇÏ´Â ´ÜÀϼ¼Æ÷·ÎÀÇ ºÐ¸®, ºÐ¼®ÇÏ°íÀÚ ÇÏ´Â ¼¼Æ÷ÀÇ ¼±Åà ¹× »ùÇøµ¿¡ ´ëÇÏ¿© ¾Ë¾Æº¸°í,
³ª¾Æ°¡ Â÷¼¼´ë ½ÃÄö½Ì(NGS)À» ÅëÇØ ¸¹Àº ¼öÀÇ ´ÜÀϼ¼Æ÷ÀÇ ºÐ¼®ÀÌ °¡´ÉÇÑ ICELL8TM ½Ã½ºÅÛ¿¡ ´ëÇÏ¿© ¼³¸íÇÕ´Ï´Ù.
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[2017 ¿Â¶óÀμ¼¹Ì³ª] Single cell ¿ÏÀü Á¤º¹À» À§ÇÑ SMART-seq¢ç (A suggestion for RNA-Seq, DNA-Seq from single cells)
¿©·¯ºÐÀº ¼¼Æ÷ ÇϳªÇϳª°¡ ¸ðµÎ ´Ù¸¥ Ư¼ºÀ» °¡Áö°í ÀÖ´Ù´Â °Í, ¾Ë°í °è½Å°¡¿ä? ÃÖ±Ù ¿¬±¸°á°ú¿¡ µû¸£¸é º¸´Ù Á¤È®ÇÑ ¿¬±¸¸¦ À§Çؼ´Â
¿©·¯ ¼¼Æ÷ÀÇ Æò±Õ°ªÀÌ ¾Æ´Ï¶ó ´ÜÀϼ¼Æ÷ °¢°¢ÀÇ Æ¯¼ºÀ» ºÐ¼®ÇÏ´Â °ÍÀÌ È¿°úÀûÀ̶ó ÇÕ´Ï´Ù.
º» ¼¼¹Ì³ª¿¡¼´Â Single cell NGS ºÐ¼® °úÁ¤¿¡¼ÀÇ ¾î·Á¿î Á¡°ú ÀÌ ¸ðµç °ÍÀ» ±Øº¹ÇÒ ¼ö ÀÖ´Â SMART-seq¢ç, Rubicon PicoPLEX¢ç
±â¼ú¿¡ ´ëÇØ ¼Ò°³ÇÕ´Ï´Ù. ´ÙÄ«¶ó¿Í ÇÔ²² Single cell ºÐ¼®À» ¿ÏÀü Á¤º¹Çϼ¼¿ä! That's Good Science!
- Single cell RNA-SeqÀÇ Á¤¼®, SMART-Seq¢ç v4 Ultra Low Input RNA Kit
- PGS, PGD, CNV ºÐ¼®ÀÇ Gold standard, Rubicon PicoPLEX¢ç DNA-Seq
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[2017 ¿Â¶óÀμ¼¹Ì³ª] °í¼øµµÀÇ ´Ü¹éÁú, Ç×ü ÀǾàÇ° »ý»êÀ» À§ÇÑ Â÷¼¼´ë Á¤Á¦ ±â¼ú_Capturem
´Ü¹éÁúÀǾàÇ°, Ç×üÀǾàÇ°À¸·Î ´ëÇ¥µÇ´Â ¹ÙÀÌ¿ÀÀǾàÇ°¿¡ ´ëÇÏ¿© ¾ó¸¶³ª ¾Ë°í °è½Å°¡¿ä? ÀǾàÇ° »ý»ê¿¡ ÇÊ¿äÇÑ ´Ü¹éÁú°ú
Ç×ü´Â ¿¬±¸ ´Ü°èºÎÅÍ Â÷º°È µÇ¾î¾ß ÇÕ´Ï´Ù. ÃÊ°í¼øµµÀÇ ´Ü¹éÁúÈ Ç×ü ÀǾàÇ° (¿¹, Antibody-Drug Conjugate: ADC)À»
À§ÇØ ¾î¶² °ÍÀÌ ÇÊ¿äÇÒ±î¿ä? º» ¿Â¶óÀÎ ¼¼¹Ì³ª¿¡¼´Â Â÷¼¼´ë ´Ü¹éÁú/Ç×ü Á¤Áö ±â¼úÀÎ Capturem¢âÀ» ¼Ò°³ÇÕ´Ï´Ù.
Next Gen Capturem¢â Technology
- ELRIC Recombinant Protein Technology 2016 meeting¿¡¼ ½Å±â¼ú/½ÅÁ¦Ç° »óÀ» ¼ö»óÇÑ ¹Ù·Î ±× ±â¼ú!
- ±âÁ¸ÀÇ Resin Á¤Á¦ ¹æ½ÄÀÌ ¾Æ´Ñ, Â÷º°ÈµÈ Membrane columnÀ» ÀÌ¿ëÇÏ¿© ½Ç¿Â¿¡¼ 5ºÐ¸¸¿¡ Á¤Á¦
- Small bed volume membraneÀ» ÀÌ¿ëÇÏ¿© ÃÊ °í¼øµµÀÇ ´Ü¹éÁú°ú Ç×ü¸¦ Á¤Á¦
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[2017 ¿Â¶óÀμ¼¹Ì³ª] ´Ü¹éÁú ´ë·®¹ßÇöÀ» À§ÇÑ È¿À²ÀûÀÎ ½Ã½ºÅÛ - Baculovirus & CHO Expression System
Àü¼¼°èÀûÀ¸·Î »õ·Î¿î ¹Ì·¡ ¼ºÀ嵿·ÂÀ¸·Î ÁÖ¸ñ¹Þ°í ÀÖ´Â ¹ÙÀÌ¿ÀÀǾàÇ°,
±× Áß¿¡¼µµ ´Ü¹éÁúÀǾàÇ°ÀÇ ¿¬±¸°³¹ß ¹× ´ë·®¹ßÇöÀ» À§ÇÑ È¿À²ÀûÀÎ Baculovirus, CHO cell Expression SystemÀ» ¼Ò°³ÇÕ´Ï´Ù.
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