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14-30 ssRNA Ladder Marker

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Takara
3416
14-30 ssRNA Ladder Marker
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62.5 ¥ìl
( 25 lane ºÐ)
443,000¿ø  3416_DS.v2310Da.pdf

Á¦Ç°¼³¸í
º» Á¦Ç°Àº 14~30 baseÀÇ 5°³ÀÇ ´ÜÀÏ°¡´Ú RNA ´ÜÆí 5°³·Î ±¸¼ºµÇ¾î ÀÖ´Ù.

´ÜÆí »çÀÌÁî(base)£º30, 26, 22, 18, 14

1ȸÀÇ Àü±â¿µµ¿¿¡ º» Á¦Ç° 2.5§¡¸¦ »ç¿ëÇÑ´Ù. ÷ºÎµÈ Loading Buffer¿Í Dye¸¦ ³Ö°í, 15% acrylamide£ºbisacrylamide(19£º1) /7M Urea/0.5¡¿TBE °Ö¿¡ »ç¿ëÇÑ´Ù. Àü±â¿µµ¿¿ë ¹öÆÛ´Â 0.5¡¿TBE¸¦ ÀÌ¿ëÇÏ°í, ¿°»ö¿¡´Â SYBR Green II¸¦ »ç¿ëÇÑ´Ù.
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RNase free water
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miRNA ȤÀº 30 base ÀÌÇÏÀÇ ssRNA Àü±â¿µµ¿¿¡ ssRNA size marker
÷ºÎ ½Ã¾à
1. RNA Loading Buffer (°³ºÀ ÈÄ´Â -20¡Éº¸Á¸ °¡´É)
95% Formamide
20 mM EDTA

2. 6¡¿Dye (°³ºÀ ÈÄ´Â 4¡Éº¸Á¸ °¡´É)
0.5% Orange G
1 mM EDTA

Keyword :

RNAi °ü·Ã Kit
>
dsRNA MW Standard Marker
siRNA Ladder Marker