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È¿¸ð·Î ºÎÅÍ °í¼øµµÀÇ Genomic DNA ÃßÃâ

Dr. GenTLE¢â (from Yeast) High Recovery

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Takara
9082
Dr. GenTLE¢â (from Yeast) High Recovery
°ü·ÃÇмú ±¸¸ÅÇϱâ
30 ȸ
211,000¿ø  9082_e.v1701Da.pdf

ÀÌ Á¦Ç°Àº Dr. GenTLE(from Yeast) (Code 9084)ÀÇ ´ëüǰÀÔ´Ï´Ù.
Á¦Ç° ¼³¸í
Dr. GenTLE™ (from Yeast) High Recovery´Â È¿¸ð¿¡ ¼¼Æ÷º® ºÐÇØÈ¿¼Ò¸¦ ó¸®ÇÏ°í, ¿°À» Á¦°ÅÇÏ¿© È¿°úÀûÀ¸·Î gDNA¸¦ ÃßÃâ ¹× Á¤Á¦ÇÑ´Ù. ½ÇÇèÀº ¿ø½ÉºÐ¸®¸¦ ÅëÇÑ È¿¸ð ±Õü ȸ¼ö, GenTLE™ Yeast Solution A (¼¼Æ÷º® ºÐÇØ È¿¼Ò Æ÷ÇÔ)¸¦ »ç¿ëÇÑ ¼¼Æ÷º® ºÐÇØ, GenTLE™ Yeast Solution B¸¦ »ç¿ëÇÑ ¼¼Æ÷ ¿ëÇØ, GenTLE™ Yeast Solution C ÷°¡ ÈÄ ¿ø½ÉºÐ¸®¸¦ ÅëÇÑ ´Ü¹éÁú ºÒ¼ø¹° Á¦°Å, ¸¶Áö¸·À¸·Î Isopropanol ħÀüÀ» ÅëÇÑ gDNAȸ¼öÀÇ ¼ø¼­·Î ÁøÇàµÈ´Ù.

ÀÌ Á¦Ç°À» »ç¿ëÇÏ¸é ¾Æ·¡ÀÇ Ç¥ (A, B)¿Í °°Àº ´Ù¾çÇÑ Á¾·ùÀÇ È¿¸ð¿¡¼­ ÀϹÝÀûÀÎ À¯Àü°øÇÐ ½ÇÇè¿¡ »ç¿ë °¡´ÉÇÑ gDNA¸¦ Á¤Á¦ÇÒ ¼ö ÀÖ´Ù. ÀϹÝÀûÀÎ ½ÇÇèÀÇ °æ¿ì 1~2 × 108 È¿¸ð ±Õü¿¡¼­ 4~10 §¶ÀÇ °íºÐÀÚ gDNA (35 ~ 200 kb)°¡ Á¤Á¦µÈ´Ù.

A

DNA ÃßÃâÀÌ °¡´ÉÇÑ È¿¸ð

Ashbya, Candida, Debaryomyces, Endomyces, Eremothecium, Hanseniaspora, Hansenula, Kloeckera, Kluyveromyces, Lipomyces, Metschikowia, Pullularia, Saccharomyces, Saccharomycopsis, Saccharomycodes, Schizosaccahromyces, Selenozyma, Trigonopsis, Wickerhamia

B

ÀϺΠstrain¿¡¼­´Â DNAÃßÃâÀÌ ¾î·Á¿î È¿¸ð

Bretanomyces, Cryptococcus, Nadsonia, Pichia, Rodosporidium, Schwanniomyces, Stephnoascus, Torulopsis

C

DNA ÃßÃâÀÌ ¾î·Á¿î È¿¸ð

Bullera, Pityrosporum, Rhosotorula, Sporidiobolus, Sporobolomyces, Stetigmatomyces, Trichosporon

³»¿ë
GenTLE¢â Yeast Solution A*1 15 ml
GenTLE¢â Yeast Solution B*2 3 ml
GenTLE¢â Yeast Solution C 6 ml
4. TE Buffer 6 ml

*1 GenTLE¢â Yeast Solution A´Â È¿¼Ò°¡ Æ÷ÇԵǾî ÀÖÀ¸¹Ç·Î ½ÉÇÑ vortexing µîÀ¸·Î È¿¼Ò°¡ ½ÇÈ°µÇÁö ¾Êµµ·Ï ÁÖÀÇÇÑ´Ù.
*2 GenTLE¢â Yeast Solution B¿¡ ħÀüÀÌ ÀÖÀ» °æ¿ì 37¡É¿¡¼­ ¿ÏÀüÈ÷ ¿ëÇØÇÑ ÈÄ »ç¿ëÇÑ´Ù.
º¸Á¸ 4¡É
GenTLE™ Yeast Solution BÀº ½Ç¿Â º¸Á¸°¡´É
Àú¿ÂÀ¸·Î º¸Á¸ÇßÀ» °æ¿ì, ħÀüÀÌ »ý±æ ¼ö ÀÖ´Ù. ÀÌ °æ¿ì 37¡É¿¡¼­ ¿ÏÀüÈ÷ ¿ëÇØÇÑ ÈÄ »ç¿ëÇÑ´Ù.

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È¿¸ð·Î ºÎÅÍ °í¼øµµÀÇ Genomic DNA ÃßÃâ
Dr. GenTLE¢â (from Yeast) High Recovery