다카라코리아바이오메디칼

Home > 전제품보기 > Transfection 시약 > 도입물질에 따른 도입시약 > TransIT®-Oligo Transfection Reagent

TransIT®-Oligo Transfection Reagent

제조사	제품코드	제품명	용량	가격 (부가세별도)	비고
Mirus	MIR 2164	TransIT-Oligo Transfection Reagent	0.4 ml	가격문의	11.01 ~ 12.27
Mirus	MIR 2160	TransIT-Oligo Transfection Reagent	1 ml	가격문의	11.01 ~ 12.27
Mirus	MIR 2165	TransIT-Oligo Transfection Reagent	1 ml×5	가격문의	11.01 ~ 12.27
Mirus	MIR 2166	TransIT-Oligo Transfection Reagent	1 ml×10	가격문의	11.01 ~ 12.27

Product index

Overview
다양한 세포/범용적인 도입..
효율증대를 위한 특수도입시..
세포에 따른 효율적인 유전..
Electroporation 관련
- Ingenio® solution & k..
- Ingenio® cuvette, 소..
도입물질에 따른 도입시약
in-vivo delivery 시스템
- TransIT Hydrodynamic D..
독성 제거 - Transfection ..
- MiraCLEAN® Endotoxin ..
siRNA Transfection 시약
siRNA 발현 · 분석
- pSINsi vector 시리즈
- Synthetic siRNA Quanti..
small RNA Cloning
RNAi 관련 Kit
- 14-30 ssRNA Ladder Mar..
- siRNA Ladder Marker

Unique Formulation - Maximize transfection performance of oligonucleotides into a wide range of cells.
Low Cellular Toxicity - Maintain cell density and reduce experimental biases.
High Efficiency Delivery - Achieve high transfection efficiency in cells to ensure experimental success.

Figure 1. The TransIT-Oligo Reagent Achieves High Transfection Efficiency. HeLa cells transfected using TransIT-Oligo Reagent and Label IT Cy™3 and Label IT Fluorescein labeled phosphothioate DNA oligos in complete media for 24 hours.

Figure 2. The TransIT-Oligo Reagent Effectively Transfects a 2'OMe RNA Oligo that Blocks a Cryptic Splice Site. The HeLa-Luc 705 reporter cell line (Kang et al. 1998, 37:6235) used in this study contains a luciferase reporter construct that has the ß-globin 705 intron inserted into the luciferase ORF. A mutation present at position 705 of the ß-globin intron activates two cryptic splice sites within the intron that lead to the production of a spliced luciferase mRNA that is disrupted by a small intron with an in-frame stop codon, thus preventing translation of functional luciferase protein. The transfection of a 2'OMe oligonucleotide complementary to the cryptic 705 splice site inhibits splicing at the cryptic splice sites enabling the complete removal of the ß-globin intron and production of a mRNA with a complete, uninterrupted luciferase ORF.

The HeLa-Luc 705 cell line was transfected with increasing amounts of the anti-705 splice site 2'OMe RNA oligo at the indicated final concentrations using the TransIT-Oligo Transfection Reagent. The cells were harvested 24 hours post-transfection and assayed for luciferase activity. The increase in luciferase activity indicates effective delivery of the anti-705 splice site RNA oligo using the TransIT-Oligo Reagent.

Keyword : V2160,MIR2160,V2164,MIR2164,V2165T,MIR2165,V2166,MIR2166

도입물질에 따른 도입시약

Large RNA & CRISPR guide RNA용transfection ..

TransIT®-mRNA Transfection Kit

TransIT®-Oligo Transfection Reagent

mRNA, sgRNA, siRNA등 다양한 RNA transfection..

Xfect™ RNA Transfection Reagent

Xfect™ Protein Transfection Reagent