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TaKaRa
Á¦Ç°¸í/Á¦Ç°ÄÚµå
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  • ÀüÈ­¹øÈ£¦¢02-2081-2510
  • Email     ¦¢[email protected]
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  • ¾÷¹«¾È³»¦¢´ëÀü/ÃæûÁö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢042-828-6525
  • Email     ¦¢[email protected]
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  • [ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
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¼¼Æ÷ ³» Localization vector

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
632453
pAcGFP1-Actin Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
928,800¿ø 
1,161,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33231
Clontech
632490
pAcGFP1-Endo Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
928,800¿ø 
1,161,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33237
Clontech
632511
pAcGFP1-F Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
928,800¿ø 
1,161,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33239
Clontech
632510
pAcGFP1-F Hyg Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
928,800¿ø 
1,161,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33238
Clontech
632464
pAcGFP1-Golgi Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
928,800¿ø 
1,161,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33240
Clontech
632491
pAcGFP1-Mem Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
928,800¿ø 
1,161,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33245
Clontech
632509
pAcGFP1-Mem Hyg Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
928,800¿ø 
1,161,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33244
Clontech
632432
pAcGFP1-Mito Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
928,800¿ø 
1,161,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33246
Clontech
632431
pAcGFP1-Nuc Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
928,800¿ø 
1,161,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33251
Clontech
632488
pAcGFP1-Tubulin Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
928,800¿ø 
1,161,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33253
Clontech
632409
pDsRed2-ER Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
928,800¿ø 
1,161,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
632421
pDsRed2-Mito Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
928,800¿ø 
1,161,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33319
Clontech
632408
pDsRed2-Nuc Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
928,800¿ø 
1,161,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33321
Clontech
632418
pDsRed2-Peroxi Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
928,800¿ø 
1,161,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33322
Clontech
632479
pDsRed-Monomer-Actin Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
928,800¿ø 
1,161,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33331
Clontech
632493
pDsRed-Monomer-F Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
928,800¿ø 
1,161,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33335
Clontech
632514
pDsRed-Monomer-F Hyg Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
928,800¿ø 
1,161,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33334
Clontech
632480
pDsRed-Monomer-Golgi Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
928,800¿ø 
1,161,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33336
Clontech
632512
pDsRed-Monomer-Mem Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
928,800¿ø 
1,161,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33340
Clontech
632513
pDsRed-Monomer-Mem Hyg Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
928,800¿ø 
1,161,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33339
Clontech
632434
pHcRed1-Mito Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
928,800¿ø 
1,161,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33382
Clontech
632433
pHcRed1-Nuc Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
928,800¿ø 
1,161,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33384
Clontech
632515
pIRES2-AcGFP1-Nuc Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
928,800¿ø 
1,161,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33399
Clontech
632583
pAutophagSENSE Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
Each
900,000¿ø 
1,125,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33273


  • »ì¾Æ ÀÖ´Â ¼¼Æ÷³» ƯÁ¤ ±â°ü°ú ±¸Á¶¿¡ ƯÀÌÀûÀ¸·Î À§Ä¡ÇÏ´Â Çü±¤(fluorescence)
  • »ý¹°ÇÐÀû ÇÁ·Î¼¼½º¸¦ ½Ã°¢È­ °¡´É
  • ¿©·¯ °³ÀÇ Çü±¤ ´Ü¹éÁúÀ» ÀÌ¿ëÇØ ¼¼Æ÷ÀÇ multiplex labeling °¡´É
Çü±¤ ´Ü¹éÁú·Î »ì¾ÆÀÖ´Â ¼¼Æ÷ ±â°ü°ú ¼¼Æ÷ ³»ºÎ¸¦ ¶óº§¸µ ÇÒ ¼ö ÀÖ´Ù.
Çü±¤ ´Ü¹éÁú º¤Å͸¦ ÀÌ¿ëÇϸé È­Çп°»ö ¾øÀÌ »ì¾ÆÀÖ´Â ¼¼Æ÷ÀÇ ³»ºÎ¿Í ±¸Á¶, ±×¸®°í ±â´É µîÀ» Çü±¤Çö¹Ì°æÀ¸·Î Á÷Á¢ÀûÀÌ°í ºñħ½ÀÀû ¹æ¹ýÀ¸·Î °üÂû ÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ ¸ñÀû ´Ü¹éÁú°ú ±¸Á¶¸¦ ¶óº§¸µÇÏ´Â ºÐ¸®µÈ Çü±¤ ´Ü¹éÁúµéÀ» ÅëÇØ ¼¼Æ÷³» ±¸Á¶¿Í °ü·Ã ÀÖ´Â ¸ñÀû ´Ü¹éÁúÀÇ À§Ä¡¸¦ ¸ð´ÏÅÍ ÇÒ ¼öµµ ÀÖ´Ù. Subcellular Localization Vector´Â ¼¼Æ÷³» ƯÁ¤ ±â°üÀ̳ª ±¸Á¶¸¦ Ÿ°ÙÀ¸·Î ÇÏ´Â Çü±¤´Ü¹éÁúÀ» ÄÚµùÇÏ¸ç ´Ù¾çÇÑ »ö±òÀ» Áö¿øÇÏ°í Àֱ⠶§¹®¿¡ multiplex labeling ½ÇÇè¿¡ ÀÌ»óÀûÀÌ´Ù.
Autophagy Sensor
pAutophagSENSE º¤ÅÍ´Â ³ì»ö Çü±¤ ´Ü¹éÁúÀÎ AcGFP1°ú mouse LC3 ´Ü¹éÁúÀÇ À¶ÇÕÀ» ¾ÏȣȭÇÏ´Â Æ÷À¯·ù ¹ßÇö º¤ÅÍÀÌ´Ù. ¼¼Æ÷³» autophagy(ÀÚ½Ä(í»ãÝ) ÀÛ¿ë)ÀÌ ÀϾÁö ¾ÊÀ» ¶§´Â ÇüÁúÀüȯµÈ ¼¼Æ÷ÀÇ ¼¼Æ÷Áú¿¡ AcGFP1-LC3 À¶ÇÕ ´Ü¹éÁúÀÌ ±ÕÀÏÇÏ°Ô ºÐÆ÷ÇÑ´Ù. ±×·¯³ª ¼¼Æ÷°¡ autophagosomeÀ» Çü¼ºÇÏ¿© autophagy¸¦ °Þ°Ô µÇ¸é AcGFP1-LC3 À¶ÇÕ ´Ü¹éÁúÀÌ autophagosomeÀ¸·Î ¼¯ÀδÙ. ¼¼Æ÷Áú¿¡¼­ autophagosomeÀÌ Çü¼ºµÇ´Â °ÍÀ» ³ì»ö Çü±¤ À¶ÇÕ ´Ü¹éÁúÀÇ ¹è¿­ º¯È­¸¦ ÅëÇØ ¸ð´ÏÅÍ ÇÒ ¼ö ÀÖ´Ù. ÀÌ ³ì»ö Çü±¤ÀÇ º¯È­´Â autophagosomeÀÇ Çü¼ºÀ» ´ëº¯ÇÑ´Ù.



Use of AcGFP1 for fusions and fluorescence microscopy applications. Panels A and B.
Activation of Protein Kinase C alpha was monitored with Living Colors AcGFP1. Panel A. HEK 293 cells were stably transfected with a plasmid encoding AcGFP1 fused to PKC alpha. Panel B. Cells were induced with 1.5 ¥ìg/ml PMA for 3 min. The PKC alpha-AcGFP1 fusion moves from the cytosol to the plasma membrane, a result consistent with the known mobilization pattern of PKC alpha. Panel C. HeLa cells were transiently transfected with pAcGFP1-Actin and visualized by fluorescence microscopy.



Keyword : Autophagy