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±¤º¯È¯Çü Çü±¤ ´Ü¹éÁú vector

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
632544
pDendra2 Vector
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 ¥ìg
900,800¿ø 
1,126,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33312, x32904
Clontech
632545
pDendra2-N Vector
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 ¥ìg
900,800¿ø 
1,126,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33314, x32904
Clontech
632546
pDendra2-C Vector
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 ¥ìg
900,800¿ø 
1,126,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33313, x32904


  • Green¿¡¼­ red·Î »öÀÌ º¯ÇÏ´Â fluorescent protein
  • Ź¿ùÇÑ À¶ÇÕ ´É·Â
  • Green°ú red »çÀÌÀÇ ¶Ñ·ÇÇÑ Â÷ÀÌ (~4,000 ¹è)
Dendra2´Â octocoral Dendronephthya sp. ¿¡¼­ À¯µµµÈ monomer·Î green¿¡¼­ redÀ¸·Î »öÀÌ º¯ÇÏ´Â fluorescent proteinÀÌ´Ù. Dendra2´Â È°¼º ´Ü¹éÁú(red)ÀÇ À̵¿°ú ºñÈ°¼º ´Ü¹éÁú(green)Çü¼ºÀ¸·Î º¯È­ ÇÏ´Â °ÍÀ» µ¿½Ã¿¡ ÃßÀûÇÒ ¼ö ÀÖ´Â ¼ö´ÜÀ» Á¦°øÇÑ´Ù. µû¶ó¼­, Dendra2´Â ´Ü¹éÁú ¿ªÇÐ (À̵¿, ºÐÇØ, µî)À» ÃßÀûÇÏ°í ¼±ÅÃÀûÀÎ ¼¼Æ÷ÀÇ ¿î¸íÀ» ½Ç½Ã°£À¸·Î °üÂûÇϱ⿡ ÀÌ»óÀûÀÌ´Ù (1-3).Dendra2´Â À¶ÇÕÀ» À§ÇÏ¿© ÀûÇÕÇÏ´Ù (Figure 1).

Dendra2´Â 20¡É¿Í 37¡É ¸ðµÎ¿¡¼­ È¿°úÀûÀ¸·Î ¼º¼÷Çϸç, ´Ù¾çÇÑ ¹üÀ§ÀÇ ½ÇÇè¿¡ Àû¿ë °¡´ÉÇÏ´Ù. Dendra2·Î transient transfection µÈ Æ÷À¯·ù ¼¼Æ÷´Â transfection ÈÄ 10~12 ½Ã°£ µ¿¾È ÀÀÁý¾øÀÌ µ¿ÀÏÇÏ°Ô ºÐÆ÷µÈ Çü±¤ signalÀ» ¹ß»êÇÑ´Ù. ¶ÇÇÑ ¾î¶°ÇÑ ¼¼Æ÷ µ¶¼ºµµ °üÂûµÇÁö ¾Ê¾Ò´Ù.


Figure 1. Green-to-red photoconversion of Dendra2-tagged proteins. HeLa cells were transiently transfected with vectors encoding Dendra2-tagged fusion proteins. Dendra2 was converted to the red state in selected cells by brief illumination with a 488 nm laser and imaged with a confocal microscope in the green and red channels.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
References
1. Gurskaya, N. G., et al. (2006) Nat. Biotechnol. 24(4):461-465.
2. Chudakov, D. M., Lukyanov, S., and Lukyanov, K. A. (2007) Nat. Protoc. 2(8):2024-2032.
3. Zhang, L., et al. (2007) Biotechniques 42(4):446, 448, 450.

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