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Stem Cell
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Mouse stem cell °ü·Ã Á¦Ç°
> Cellartis¢ç 2i mESC/iPSC Culture Medium
Mouse ESC/iPSC ¹è¾ç¹èÁö (2i Technology)
Cellartis¢ç 2i mESC/iPSC Culture Medium
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Cellartis
Y40031
Cellartis¢ç 2i mES/iPSC Culture Medium
200 §¢
(Package)
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Overview
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Mouse stem cell °ü·Ã Á¦Ç°
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RHB-A/RHB Basal
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Coating matrix
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Feeder-free, Serum-free, LIF-free »óÅ¿¡¼ ¡¯Ground State (Naive state)¡¯ »óÅ·Πmouse iPSC/ESC ¹è¾ç
GSK3¥â, ERK/MEK inhibitor (2i)¸¦ »ç¿ëÇÏ¿© mES/iPSCÀÇ ºÐÈ À¯µµ ½ÅÈ£ ¾ïÁ¦ ¹× ¼ºÀå ÃËÁø
LIF ÷°¡·Î mouse partial iPSC ¹× pre-iPSC¸¦ ¿ÏÀüÇÑ pluripotent iPS ¼¼Æ÷·Î º¯È¯ °¡´É
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Cellartis
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2i mES/iPSC Culture MediumÀº 2Á¾·ùÀÇ small molecule inhibitor (CHIR99021, PD0325901= '2i')¸¦ »ç¿ëÇÏ¿©, mouse ESC, iPSC¸¦ Á¦ÀÛÇϱâ À§ÇÑ defined, serum free ¹è¾ç ¹èÁöÀÌ´Ù. CHIR99021, PD0325901Àº °¢°¢ GSK3 ¥â ¶Ç´Â ERK/MEKÀÇ ºÐÈ À¯µµ ½ÅÈ£¸¦ ¾ïÁ¦ÇÏ¿© ¼¼Æ÷ÀÇ ¼ºÀåÀ» ÃËÁøÇÑ´Ù. ¶ÇÇÑ, LIF¸¦ ÷°¡ÇÔÀ¸·Î½á, mouse partial iPSC³ª pre-iPSC¸¦ ¿ÏÀüÇÑ pluripotent iPSC·Î º¯È¯µµ °¡´ÉÇÏ´Ù. (Âü°í ¹®Çå 1, 2)
Cellartis
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2i mES/iPSC Culture Medium¸¦ »ç¿ëÇϸé Ground State (Naive state)·Î mouse ESC/iPSC¸¦ Àå±â°£ À¯ÁöÇÒ ¼ö ÀÖ´Ù.
2i technology´Â »ý½Ä °è¿ (germline-competent)·Î ºÐÈ °¡´ÉÇÑ ESC¸¦ ¾ò±â ¾î·Á¿ü´ø mouse strain¿¡¼ ESC¸¦ ¹è¾ç °¡´ÉÇÏ°Ô ÇØÁÖ¸ç, human°ú livestockÀÇ iPSCÀÇ Á¦ÀÛ¿¡µµ ÀÀ¿ëÇÒ ¼ö ÀÖ´Ù.
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Cellartis mES/iPSC Culture Basal Medium (Code Y40005, 200 §¢; º°µµ±¸¸Å ºÒ°¡)
Cellartis 2i mES/iPSC Supplement (Code Y40032, 200 §¡; º°µµ±¸¸Å ºÒ°¡)
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Cellartis mES/iPSC Culture Basal Medium : -20 ¡É
Cellartis 2i mES/iPSC Supplement : -80 ¡É
»ç¿ë»ó ÁÖÀÇ»çÇ×
°í¿Â ´Ù½ÀÇÑ È¯°æÀ» ÇÇÇÏ°í, Â÷±¤ÇÏ¿© º¸°üÇϽʽÿÀ.
Çص¿ ÈÄ Basal medium¿¡ Supplement¸¦ Àü·® ÷°¡ÇÏ°í, 4 ¡É¿¡ º¸°üÇϸç 2ÁÖÀÏ À̳»¿¡ »ç¿ëÇϽʽÿÀ. (Do not refreeze after thawing)
»ç¿ëÀü Á¦Á¶ÇÑ Cellartis
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2i mES/iPSC Culture MediumÀº »ç¿ëÇÒ ¾ç¸¸ ¼ÒºÐÇÏ¿© µû¶æÇÏ°Ô ÇÑ ÈÄ »ç¿ëÇϽʽÿÀ. (warm this aliquot to between room temperature and 37¡É)
Âü°í ¹®Çå
1. Silva J
et al
.
Promotion of Reprogramming to Ground State Pluripotency by Signal Inhibition
.
PLoS Biol
. (2008)
6
: e253.
2. Silva J
et al
.
Nanog is the Gateway to the Pluripotent Ground State
.
Cell
. (2009)
138
(4): 722-737.
3. Nichols J
et al
.
Validated germline-competent embryonic stem cell lines from non-obese diabetic mice.
Nat Med
. (2009)
15
: 814-818.
4. Wang W
et al
.
Rapid and efficient reprogramming of somatic cells to induced pluripotent stem cells by retinoic acid receptor gamma and liver receptor homolog
.
PNAS (USA)
. (2011)
108
(45): 18283-18288.
5. Malaver-Ortega LF
et al
.
The state of the art for pluripotent stem cells derivation in domestic ungulates
.
Theriogenology
. (2012)
78
(8):1749-1762.
Keyword :
Mouse stem cell °ü·Ã Á¦Ç°
[Àû¿ë] NDiff 227À» ÀÌ¿ëÇÑ mouse organoid Á¦ÀÛ
Mouse ESC/iPSC ¹è¾ç¹èÁö (3i technology)
Cellartis¢ç 3i mESC/iPSC Culture Medium
Mouse ESC/iPSC ¹è¾ç¹èÁö (2i Technology)
Cellartis¢ç 2i mESC/iPSC Culture Medium
È¿À²ÀûÀÎ ¸¶¿ì½º/ human ½Å°æÁٱ⼼Æ÷ÀÇ À¯Áö¿Í..
RHB-A/RHB Basal
¸¶¿ì½º ES ¼¼Æ÷ ½Å°æºÐȹèÁö
NDiff227
Human, mouse ES ¼¼Æ÷¿Í ½Å°æ Áٱ⼼Æ÷ ¹è¾ç¿¡ ..
NDiff N2
°ñ¾Æ¼¼Æ÷ ºÐÈ ½Ã¾à
Osteoblast-Inducer Reagent (for animal cell)