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BamH I

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
1010A
BamH I
°ü·ÃÇмú ±¸¸ÅÇϱâ
10,000 U
83,000¿ø  1010A_DS.v1807Da.pdf
Takara
1010B
BamH I
°ü·ÃÇмú ±¸¸ÅÇϱâ
50,000 U (1010A x 5)
375,000¿ø 
Takara
1010AH
BamH I (°í³óµµ)
°ü·ÃÇмú ±¸¸ÅÇϱâ
10,000 U
83,000¿ø  1010AH_DS.v1807Da.pdf
Takara
1010BH
BamH I (°í³óµµ)
°ü·ÃÇмú ±¸¸ÅÇϱâ
50,000 U (1010AH x 5)
375,000¿ø 

º¸Á¸
-20¡É
³óµµ
15 U/§¡ [°í³óµµ: 50 U/§¡]
÷ºÎ ¹× È°¼º ÃøÁ¤ buffer
Kbuffer
[Âü°í] Universal Buffer ¡¤ Basal Buffer¿¡ ÀÇÇÑ Á¦ÇÑÈ¿¼Ò È°¼º Ç¥½Ã ½Ã½ºÅÛ
[Âü°í] Double Digestion¿ë Ãßõ Universal Buffer
¹ÝÀÀ ¿Âµµ
30¡É
È°¼ºÀ» ÃøÁ¤ÇÑ ±âÁú
¥ëDNA
±â¿ø
Escherichia coli carrying the plasmid encoding BamHI gene
¸ÞÆ¿È­ÀÇ ¿µÇâ
dam methylase, dcm methylase¹× CG methylaseÀÇ ¿µÇâÀ» ¹ÞÁö ¾Ê´Â´Ù.
Star È°¼º
°í³óµµÀÇ ±Û¸®¼¼·Ñ, DMSO ¶Ç´Â Mn2+°¡ Á¸ÀçÇÒ ¶§, ¼­¿­ ÀÎ½Ä Æ¯À̼ºÀÌ °¨¼ÒµÉ ¼ö ÀÖ´Ù.
»ç¿ë »óÀÇ ÁÖÀÇ
37¡É ¹ÝÀÀ¿¡¼­µµ µ¿µîÇÑ È¿¼Ò È°¼ºÀ» ³ªÅ¸³»Áö¸¸ 30¡É¿¡¼­ ¹ÝÀÀÇÏ´Â °Í¿¡ ºñÇØ ¾ÈÁ¤¼ºÀÌ ÀϺΠÀúÇϵȴÙ.
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