Enzyme ÷°¡ ¾ç : 1 §¡ / 50 §¡ total volume1
÷ºÎ buffer : QuickCut Buffer/ QuickCut Green Buffer2 Åà 1
¹ÝÀÀ Á¶°Ç : 37 ¡É, 5 - 15 min3
¸ÞÆ¿ÈÀÇ ¿µÇâ : CG methylase ¿µÇâÀ» ¹ÞÀ½
1Enzyme ¾çÀº »ùÇÿ¡ µû¶ó »óÀÌ ÇÒ ¼ö ÀÖÀ¸´Ï. ¸Å´º¾ó ÂüÁ¶
2QuickCut Green Buffer : loading dye°¡ Æ÷ÇԵǾî ÀÖ´Â buffer·Î ¹ÝÀÀ ÈÄ gel loading ¿ëÀÌ
3DNAÀÇ Á¾·ù³ª °íÂ÷±¸Á¶ µî¿¡ ÀÇÇØ ¹ÝÀÀ ½Ã°£ Á¶Àý ÇÊ¿ä ÇÒ ¼ö ÀÖÀ½
ÁÖÀÇ »çÇ×
ÀÏ¹Ý restriction enzyme series¿Í ȣȯ ºÒ°¡. QuickCut restriction enzyme ¹× QuickCut¿ë buffer¸¦ ÀÏ¹Ý restriction enzyme series È¥¿ëÇÏ¿© »ç¿ëÇÏÁö ¸¶½Ã±â ¹Ù¶ø´Ï´Ù.