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Cycling Probe ¹ýÀ» ÀÌ¿ëÇÑ Real Time PCR ¿ë ½Ã¾à

CycleavePCR Core Kit

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Takara
CY501
CycleavePCR Core Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
50 ȸ
139,000¿ø  CY501_k1903.pdf

* º» Á¦Ç° ¸Å´º¾óÀº ÀϹ®¸Å´º¾óÀÇ Çѱ۹ø¿ªº» ÀÔ´Ï´Ù.
°ü·ÃÇؼ­ ¹®Àdz»¿ë ÀÖÀ¸½Ã¸é °í°´Áö¿ø¼¾ÅÍ (02-2081-2510, [email protected])·Î ¹®Àǹٶø´Ï´Ù.
³»¿ë (50 ȸ)
10¡¿ CycleavePCR Buffer 125 §¡
dNTP Mixture (°¢2.5 mM) 150 §¡
Mg2+ solution (25 mM) 250 §¡
TaKaRa Ex Taq HS (5 U/§¡) 12.5 §¡
Tli RNase H II (200 U/§¡) 25 §¡
Positive Control (104 copies/§¡)*1 10 §¡
Positive Control Primer mix (°¢10 §­) 10 §¡
Positive Control probe (FAM Ç¥½Ä)*2 (25¡¿) 10 §¡
dH2O 700 §¡
*1 Positive ControlÀº Çö󽺹̵åÀ̸ç, copy¼ö´Â A260ÀÇ Èí±¤µµ·ÎºÎÅÍ È¯»êÇÑ ÁöÇ¥·Î ½ÇÁ¦ÀÇ ºÐÀÚ¼ö¸¦ ³ªÅ¸³»°í ÀÖ´Â °ÍÀº ¾Æ´Õ´Ï´Ù.
*2 ¹ÝÀÀ control °ËÃâ¿ëÀÇ ÇÁ·ÎºêÀÔ´Ï´Ù. Çü±¤ Ç¥½Ä ÇÁ·Îºê´Â Â÷±¤¿¡ À¯ÀÇÇϽñ⠹ٶø´Ï´Ù.
º¸Á¸ - 20¡É
Á¦Ç°¼³¸í
º» Á¦Ç°Àº Cycling probe¸¦ ÀÌ¿ëÇÏ´Â Real Time PCR Àü¿ë Å°Æ®ÀÌ´Ù. ½Å¼Ó¼º°ú Á¤·®¼ºÀÌ ¶Ù¾î³­ Real Time PCR ¹ý°ú ƯÀ̼ºÀÌ ¸Å¿ì ³ôÀº °ËÃâ¹ýÀÎ Cycling probe¹ýÀ» Á¶ÇÕÇÏ¿© Ÿ°ÙÀÇ °ËÃâÀ̳ª Á¤·®, SNPÀÇ °ËÃâ µîÀ» Á¤È®ÇÏ°í °£ÆíÇÏ°Ô ÇÒ ¼ö ÀÖ´Ù.
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- Real Time PCR°ú Cycling probe¹ýÀ¸·Î ½Å¼Ó, Á¤È®ÇÏ°Ô PCR ¹ÝÀÀ °á°ú¸¦ ¾Ë ¼ö ÀÖ´Ù. ÇÑ ¿°±âÀÇ Â÷À̸¦ ½Äº°ÇÏ´Â SNP Çؼ®À» À§ÇÑ °ËÃâ°è¸¦ ±¸ÃàÇÏ´Â Àϵµ °¡´ÉÇÏ´Ù.
- PCR¿¡´Â Hot Start¿ë È¿¼Ò TaKaRa Ex Taq HS¸¦ ÀÌ¿ëÇÏ°í ÀÖ´Ù. Real Time PCR ¿ëÀ¸·Î ÃÖÀûÈ­ µÇ¾î ÀÖ¾î, ÁõÆø È¿À²ÀÌ ÁÁ°í, °í°¨µµ °ËÃâÀÌ °¡´ÉÇÏ´Ù.
º» Á¦Ç° ¿Ü¿¡ ÇÊ¿äÇÑ ½Ã¾à, ±â±â (ÁÖ¿äÇÑ °Í¸¸ Ç¥±â)
1. Real Time PCR ±â±â (Authorized instruments)
    Thermal Cycler Dice Real Time System II (Code TP900/TP960)
    Smart Cycler II System (Code SC200Nµî)
    Applied Biosystems 7500 Real-Time PCR System (Life Technologies)¿Ü
2. Thermal Cycler Dice Real Time¿ë plate (Code NJ400)
    Smart Cycler tube (Code SC910A/C)¿Ü
3. PCR¿ë primer*
4. Cycling probe*
5. Micropipette ¹× tip (¸ê±Õó¸®ÇÑ °Í)

*Primer ¹× Cycling probe µðÀÚÀÎÀº ´ÙÄ«¶óÄÚ¸®¾Æ °í°´Áö¿ø¼¾ÅÍ (02-2081-2510, [email protected])·Î ¹®Àǹٶø´Ï´Ù.

Keyword :

CycleavePCR °ËÃâ
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