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Inducible System ¡¤ Yeast hybrid
>
Tet À¯µµ ¹ßÇö ½Ã½ºÅÛ
> pTet-tTS Vectors
pTet-tTS Vectors
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Clontech
631011
pTet-tTS Vector
20 ¥ìg
672,000¿ø
840,000¿ø
11.01 ~ 12.27
Product index
Tet À¯µµ ¹ßÇö ½Ã½ºÅÛ
[°³¿ä] Tet-on system
[¼±Åð¡À̵å] Tet Syste..
Tet-On 3G À¯µµ ¹ßÇö ½Ã..
Lenti-X Tet-On 3G Indu..
Tet-On 3G Cell Line
Tet Approved FBS
Tet °ü·Ã Ç×»ýÁ¦
Selection marker (Line..
Tet-One Inducible Expr..
TetR Ç×ü
[Legacy] Tetracycline ..
Tet-Off Cell Lines
pTRE-Tight Vectors
pTet-tTS Vectors
IRES Tet À¯µµ¹ßÇö ½Ã½º..
iDimerize Inducible System..
[°³¿ä] Inducible Prote..
iDimerize Antibodies
Inducible Expression S..
Heterodimer System
Homodimer System
Regulated Transcriptio..
Reverse Dimerization S..
´Ü¹éÁú ¹ßÇö·® Á¶Àý ½Ã½ºÅÛ ..
ProteoTuner System
Tet-ProteoTuner Vector..
[°³¿ä] Yeast Hybrid System
[FAQ] Matchmaker Gold ..
[°³¿ä] Matchmaker Gold..
Yeast Two Hybrid System (Y..
Y2H Library Constructi..
Yeast Culture media (T..
Yeast Two-Hybrid (Y2H)..
Y2H Library (Yeast ³»..
Normalized Y2H Library..
Yeast Two hybrid vecto..
pBridge Vector
Yeast One-Hybrid (Y1H)
Yeast Culture Media (O..
Yeast One-Hybrid (Y1H)..
Matchmaker °ü·ÃÁ¦Ç°
Yeast Culture Media
Yeast Culture Media (..
Aureobasidin A
Yeast Plasmid Isolati..
Matchmaker Ç×ü
Insert Check Kit (prey..
Yeast Transformation M..
X-¥á-Gal
Yeast Transformation S..
Mammalian Cell Assay K..
pTet-tTS (Figure 1)´Â tetracyclineÀ¸·Î Á¶ÀýµÇ´Â transcription silencer (tTS)¸¦ ¹ßÇö½ÃŲ´Ù. tTS´Â KRAB silencing domain (SD
Kid-1
)°ú Tet Repressor protein (TetR)ÀÌ À¶ÇÕµÈ ´Ü¹éÁúÀÌ´Ù (1). tTS´Â Tet-On Expression System¿¡¼ ¸ñÀû À¯ÀüÀÚÀÇ basal expressionÀ» ³·Ãß¾î ÁØ´Ù. DoxycyclineÀÌ ¾øÀ» ¶§, tTS´Â response plasmidÀÇ TRE ºÎÀ§¿¡ ÀÖ´Â tetO ¼¿°ú °áÇÕÇÏ¿© À¯ÀüÀÚ ¹ßÇöÀ» ¹æÇØÇÑ´Ù. ClontechÀº tTS¸¦ ¹ßÇöÇÏ´Â ¹Ì¸® Á¦ÀÛµÈ ´Ù¾çÇÑ Á¾·ùÀÇ cell lines¸¦ Á¦°øÇÏ°í ÀÖ´Ù.
Figure 1. Map of the pTet-tTS vector.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
References
1. Freundlieb, S. et al. (1999) J. Gene Med. 1(1):4-12.
Keyword :
Tet À¯µµ ¹ßÇö ½Ã½ºÅÛ
[°³¿ä] Tet-on system
[¼±Åð¡À̵å] Tet Systems Product
Tet-On 3G À¯µµ ¹ßÇö ½Ã½ºÅÛ
Lenti-X Tet-On 3G Inducible Expression System
Tet-On 3G Cell Line
Tet Approved FBS
Tet °ü·Ã Ç×»ýÁ¦
Selection marker (Linear / Plasmid)
All-in-one vector ÇüÅÂÀÇ Tet-inducible syste..
Tet-One Inducible Expression (One vector System)
TetR Ç×ü
[Legacy] Tetracycline À¯µµ¹ßÇö ½Ã½ºÅÛ
Tet-Off Cell Lines
pTRE-Tight Vectors
pTet-tTS Vectors
IRES Tet À¯µµ¹ßÇö ½Ã½ºÅÛ