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pTet-tTS Vectors

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Clontech
631011
pTet-tTS Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
672,000¿ø 
840,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33579

pTet-tTS (Figure 1)´Â tetracyclineÀ¸·Î Á¶ÀýµÇ´Â transcription silencer (tTS)¸¦ ¹ßÇö½ÃŲ´Ù. tTS´Â KRAB silencing domain (SDKid-1)°ú Tet Repressor protein (TetR)ÀÌ À¶ÇÕµÈ ´Ü¹éÁúÀÌ´Ù (1). tTS´Â Tet-On Expression System¿¡¼­ ¸ñÀû À¯ÀüÀÚÀÇ basal expressionÀ» ³·Ãß¾î ÁØ´Ù. DoxycyclineÀÌ ¾øÀ» ¶§, tTS´Â response plasmidÀÇ TRE ºÎÀ§¿¡ ÀÖ´Â tetO ¼­¿­°ú °áÇÕÇÏ¿© À¯ÀüÀÚ ¹ßÇöÀ» ¹æÇØÇÑ´Ù. ClontechÀº tTS¸¦ ¹ßÇöÇÏ´Â ¹Ì¸® Á¦ÀÛµÈ ´Ù¾çÇÑ Á¾·ùÀÇ cell lines¸¦ Á¦°øÇÏ°í ÀÖ´Ù.


Figure 1. Map of the pTet-tTS vector.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
References
1. Freundlieb, S. et al. (1999) J. Gene Med. 1(1):4-12.

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