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pTRE-Tight Vectors

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
631059
pTRE-Tight Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
681,600¿ø 
852,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33606, x33152
Clontech
631625
Linear Hygromycin Marker
°ü·ÃÇмú ±¸¸ÅÇϱâ
2 ¥ìg
265,600¿ø 
332,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
631626
Linear Puromycin Marker
°ü·ÃÇмú ±¸¸ÅÇϱâ
2 ¥ìg
265,600¿ø 
332,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â

pTRE-Tight´Â response plasmid·Î ÃÖÀûÈ­µÈ TRE ±â¹ÝÀÇ promoterÀÎ PTight¸¦ °¡Áö°í ÀÖ¾î Á¤¹ÐÇÏ°Ô ¸ñÀû À¯ÀüÀÚÀÇ À¯µµ ¹ßÇöÀ» Á¶ÀýÇÒ ¼ö ÀÖ´Ù(Figure 1). PTight´Â ³·Àº basal expression°ú ÃÖ°íÀÇ inducible expressionÀ» Á¦°øÇÑ´Ù. ¶ÇÇÑ, pTRE-Tight¿Í Linear Selection Marker¸¦ Tet-On ¶Ç´Â Tet-Off Advanced transactivator¸¦ ¹ßÇöÇÏ´Â ¼¼Æ÷·Î ÇÔ²² transfection ½ÃÅ°¸é ÃÖ»óÀÇ °á°ú¸¦ ¾òÀ» ¼ö ÀÖ´Ù. ¸ðµç Tet-inducible cell lines ¶Ç´Â Tet-inducible system°ú ÇÔ²² »ç¿ë °¡´ÉÇÏ´Ù.


Figure 1. Map of the pTRE-Tight inducible expression vector.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
References
1. pTRE-Tight Vectors (April 2003) Clontechniques XVIII(2):10-11.

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