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TaKaRa
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Alkaline Phosphatase (Shrimp) (SAP)

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
2660A
Alkaline Phosphatase (Shrimp)
°ü·ÃÇмú ±¸¸ÅÇϱâ
300 U
156,000¿ø  2660A_DS.v2109Da.pdf
Takara
2660B
Alkaline Phosphatase (Shrimp)
°ü·ÃÇмú ±¸¸ÅÇϱâ
1,500 U (2660A x 5)
705,000¿ø 

Á¦Ç°¼³¸í
º» È¿¼Ò´Â ´ëÀå±Õ À¯·¡ÀÇ È¿¼Ò¿Í ¸¶Âù°¡Áö·Î ´ëºÎºÐÀÇ Àλê monoester °áÇÕÀ» °¡¼öºÐÇØÇÏÁö¸¸, Àλê diester¿Í Àλê triester °áÇÕÀº ºÐÇØÇÏÁö ¾Ê´Â´Ù. ¶ÇÇÑ ´ëÀå±Õ À¯·¡ÀÇ È¿¼Ò¿Í ´Þ¸® 65¡É¿¡¼­ 15ºÐ°£ ¿­Ã³¸®ÇÏ¸é ºñ°¡¿ªÀûÀ¸·Î ½ÇÈ°ÇÑ´Ù.
º¸Á¸
-20¡É
³óµµ
1 U/§¡
Çü»ó

25 mM

 

Tris-HCl, pH7.6 (4¡É)

5 mM

 

MgCl2

50%

 

Glycerol

÷ºÎ buffer ±¸¼º (10x)

500 mM

 

Tris-HCl, pH9.0 (37¡É)

50 mM

 

MgCl2

È°¼ºÀÇ Á¤ÀÇ
p-nitrophenyl phosphate¸¦ ±âÁú·Î ÇÏ¿© 37¡É, pH9.8¿¡¼­ 1 ºÐ°£ 1 ¥ìmolÀÇ p-nitrophenolÀ» À¯¸®½ÃÅ°´Â È¿¼ÒÈ°¼ºÀ» 1 UÀ¸·Î ÇÑ´Ù.
È°¼º ÃøÁ¤¿ë ¹ÝÀÀ¾× Á¶¼º

1 M

 

Diethanolamine (pH9.8)

1 mM

 

p-nitrophenyl phosphate

5 mM

 

MgCl2

¿ëµµ
- DNAÀÇ 5¡¯-¸»´Ü Å»ÀλêÈ­ (dephosphorylation)
±â¿ø
Pichia pastoris that has been genetically modified with a gene coding a shrimp alkaline phosphatase.
ÀϹÝÀû ¼ºÁú
-ÃÖÀû pH: pH9.8
-¾ÈÁ¤¼º
65¡É, 15ºÐÀÇ ¿­Ã³¸®·Î ¿ÏÀüÈ÷ ºñ°¡¿ªÀûÀ¸·Î ½ÇÈ°ÇÑ´Ù.
°ü·Ã Á¦Ç°
- ½ÇÇè¸ñÀûº°·Î ¼±ÅÃÇÏ´Â Cloning Kit ¼±Åà °¡À̵å - ¸µÅ©
DNA Ligation Kit ¡´Mighty Mix¡µ (Code 6023)
Mighty TA-cloning Kit (Code 6028)
Mighty TA-cloning Reagent Set for PrimeSTAR¢ç (Code 6019)
Alkaline Phosphatase (E. coli C75) (Code 2120A)
Alkaline Phosphatase (Calf intestine) (Code 2250A)

Keyword :

¼ö½ÄÈ¿¼Ò£¯Alkaline Phosphatase¡¢Polynucleotide Kinase