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½Ä¹° ÇüÁúÀüȯ¿ë Binary Vector

pRI 909/910 DNA

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Takara
3260
pRI 909 DNA
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10 ¥ìg
662,000¿ø  3260_DS.v2109Da.pdf
Takara
3261
pRI 910 DNA
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662,000¿ø  3261_DS.v2109Da.pdf

* Takara Plant ½ÇÇè °ü·ÃÁ¦Ç° overview (Ŭ¸¯)
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10 mM Tris - HCl, pH 8.0 1 mM EDTA
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9,168 bp
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Rhizobium (Agrobacterium) rhizogenesÀÇ Ri plasmid À¯·¡ÀÌÁö¸¸ vir ºÎºÐÀº Æ÷ÇÔÇÏÁö ¾Ê´Â´Ù. º» vector´Â ´ëÀå±Õ ¹× Rhizobium (Agrobacterium)¿¡¼­ ÀÚÀ²ÀûÀ¸·Î º¹Á¦µÇ´Â shuttle vector·Î pUC°èÀÇ plasmid¿Í °°Àº º¹Á¦ ±âÁ¡(ColE1 ori)À» Æ÷ÇÔÇϱ⠶§¹®¿¡ ´ëÀå±Õ¿¡¼­´Â high copy plasmid·Î À¯ÁöµÇ°í, Ri plasmid À¯·¡ÀÇ º¯ÀÌÇü º¹Á¦ ±âÁ¡ (Ri-ori)À» Æ÷ÇÔÇϱ⠶§¹®¿¡ Rhizobium (Agrobacterium) ³»¿¡¼­µµ ¾ÈÁ¤ÇÏ°Ô À¯ÁöµÈ´Ù. ´ëÀå±Õ, Rhizobium (Agrobacterium)¿¡¼­ÀÇ ¼±Åø¶Ä¿·Î kanamycin³»¼ºÀ¯ÀüÀÚNPT III¸¦, ½Ä¹°¿¡¼­ÀÇ ¼±Åø¶Ä¿·Î º¯ÀÌÇü kanamycin ³»¼ºÀ¯ÀüÀÚ NPT II¸¦ Æ÷ÇÔÇÏ°í ÀÖ´Ù. Cloning site·Î pUC°èÀÇ plasmid¿¡ »ç¿ëµÇ´Â Á¦ÇÑ È¿¼Ò Àý´Ü ºÎÀ§¸¦ ÀÌ¿ëÇÒ ¼ö ÀÖ´Ù.
Rhizobium (Agrobacterium)°ú ÇÔ²² binary vector¹ý¿¡ ÀÇÇÑ ½Ä¹°ÇüÁúÀüȯ¿¡ ÀÌ¿ëÇÒ ¼ö ÀÖ°í, ¶ÇÇÑ T-DNA»ó¿¡¼­ cloning site°¡ ½Ä¹° ¼±Åø¶Ä¿ (NPT II)ÀÇ right border (RB)¿¡ ¹èÄ¡µÇ¾î Àֱ⠶§¹®¿¡ ¸ñÀû À¯ÀüÀÚ°¡ °á½ÇµÇÁö ¾Ê°í ¾ÈÁ¤ÀûÀ¸·Î ½Ä¹° ¿°»öü¿¡ »ðÀÔÀÌ °¡´ÉÇÏ´Ù.
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pRI 909/910 DNA

Keyword :

½Ä¹°¿ë Vectors
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½Ä¹° ÇüÁúÀüȯÀ» À§ÇÑ À¯ÀüÀÚ µµÀÔ¿¡
Agrobacterium tumefaciens LBA4404 Electro-Cells
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½Ä¹° ÇüÁúÀüȯ¿ë °í¹ßÇö Vector
pRI 101 DNA ½Ã¸®Áî
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½Ä¹° ÇüÁúÀüȯ¿ë °í¹ßÇö Vecto£¨New version£©
pRI 201 DNA ½Ã¸®Áî
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½Ä¹° ÇüÁúÀüȯ¿ë Binary Vector
pRI 909/910 DNA