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´Ù¾çÇÑ tag ´Ü¹éÁú °ü·Ã
> GST-tag À¶ÇÕ ´Ü¹éÁú Á¤Á¦ Resin
GST-tag À¶ÇÕ ´Ü¹éÁú Á¤Á¦ Resin
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Clontech
635607
Glutathione-Superflow Resin
10 ml
386,400¿ø
483,000¿ø
11.01 ~ 12.27
Clontech
635608
Glutathione-Superflow Resin
100 ml
3,033,600¿ø
3,792,000¿ø
11.01 ~ 12.27
Clontech
635619
GST Purification Kit
5 ȸ
901,600¿ø
1,127,000¿ø
11.01 ~ 12.27
Product index
Overview
´Ü¹éÁú ¹ßÇö½Ã½ºÅÛ - »ó..
[°³¿ä] Â÷¼¼´ë ´Ü¹éÁú ..
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
[¼±Åð¡À̵å] Protein E..
His-tag À¶ÇÕ ´Ü¹éÁú Á¤Á¦
[¼±Åð¡À̵å] His-tag ..
Capturem¢â His-Tagged ..
TALON (Cobalt) resin ..
TALON Magnetic Beads
TALON (Cobalt) Superfl..
xTractor Buffer Kit
His60 (Nickel) Superfl..
His60 Ni Magnetic Bead..
HRV 3C Protease
Poly Histidine tag Ç×..
TALON (Cobalt) CellThr..
TALONSpin Column
´Ü¹éÁú ÃßÃâ ¡¤ Á¤Á¦°ü·Ã
Calpastatin
Cryonase¢â Cold-active..
Glutathione Excellose ..
IDA Excellose (polyhis..
MBP Excellose (MBP fus..
MiniExcellose
ProteoGuard EDTA-Free ..
Streptavidin-based Enr..
Ç×ü Á¤Á¦
Ç×ü Á¤Á¦ Resin
Capturem¢â IP £¦ Co-IP..
°ïÃæ ¼¼Æ÷ ´Ü¹éÁú ¹ßÇö (Bac..
[Overview] Insect Expr..
BacPAK¢â Baculovirus G..
BacPAK¢â p35 ELISA Kit..
BacPAK Baculovirus ¹ß..
Baculovirus control vi..
Baculovirus DNA packag..
Baculovirus Titer Kit
flashBAC¢â Baculovirus..
FoldHelper Helper viru..
ProEasy Baculovirus Ve..
ProFectin
ProFold Baculovirus Ve..
qPCR Titer ÃøÁ¤ Kit
Transfer Vectors
Æ÷À¯·ù ¼¼Æ÷ ´Ü¹éÁú ¹ßÇö
[Overview] Mammalian E..
CHOgro¢ç Expression Sy..
CHOgro¢ç High Yield Ex..
Corystein¢â£¨Purothion..
Human Cell-Free Protei..
pHEK293 Ultra Expressi..
´ëÀå±Õ ´Ü¹éÁú ¹ßÇö
[Overview] Bacterial E..
´ëÀå±Õ ´Ü¹éÁú ¹ßÇö £¦ ..
Anti-ProS2
Anti-TF(Trigger Factor..
Chaperone Competent Ce..
Chaperone Plasmid Set
HAT ´Ü¹éÁú ¹ßÇö£¦ Á¤Á¦..
pCold¢â GST DNA
pCold¢â ProS2 DNA
pCold¢â TF DNA
pCold¢â Vector ½Ã¸®Áî
Single Protein Product..
TaKaRa Competent Cell ..
Brevibacillus£¯Bacillus ¹ß..
B. subtilis Secretory ..
´Ù¾çÇÑ tag ´Ü¹éÁú °ü·Ã
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
Excellose Spin Kit His..
FLAG tag ´Ü¹éÁú »ý»ê
GST-tag À¶ÇÕ ´Ü¹éÁú Á¤..
´ç£¯ÀλêÈ ´Ü¹éÁú °ü·Ã
Glycoprotein ³óÃà¿ë Re..
Magnetic bead - Phosph..
Phosphoprotein enrichm..
´Ü¹éÁú ¿°»ö ½Ã¾à
TaKaRa CBB Protein Saf..
SYPRO Protein Gel Stai..
SYPRO Ruby Protein Sta..
´Ü¹éÁú °ËÃâ, Á¤·®, Çؼ®
HRP Substrate ½Ã¸®Áî
HRP-Juice
ProSieve¢â EX Running ..
TaKaRa BCA Protein Ass..
TaKaRa Bradford Protei..
Tris-Glycine Powder
Western BLoT Blocking/..
Western BLoT Immuno Bo..
Western BLoT Immuno Bo..
Western BLoT Rapid Det..
Protease (¾Æ¹Ì³ë»ê ¼¿ ÇØ..
Arginylendopeptidase (..
Pfu Aminopeptidase I
Pfu Methionine Aminope..
Pfu N-acetyl Deblockin..
Pfu Protease S
Pfu Pyroglutamate Amin..
°í¼øµµÀÇ GST-tagged proteinÀ» one-stepÀ¸·Î Á¤Á¦
³ôÀº °áÇÕ·Â (resin 1ml ´ç 10 mg ÀÌ»óÀÇ tagged protein)
Gravity flow¿Í FPLC Àû¿ëÀ» À§ÇÏ¿© ´Ù¾çÇÑ ÇüÅ·ΠÀÌ¿ë°¡´É
¿ëÀÌÇÑ Àç»ý & ÇÕ¸®ÀûÀÎ °¡°Ý
Flexible Resin Format
Glutathione-Superflow¿Í Glutathione-Uniflow ResinÀº ³ôÀº ģȷ°ú ƯÀ̼ºÀ¸·Î GST (glutathione-S-transferase) tag°ú °áÇÕÇÏ¿© GST-tagged proteinÀ» ºü¸£°í È¿À²ÀûÀ¸·Î Á¤Á¦Çϵµ·Ï ÇÑ´Ù. ÀÌ resinÀº glutathioneÀÌ °øÀ¯ °áÇյǾî ÀÖ´Â °¢°¢ 6%¿Í 4% cross-linked agarose¿¡ ±â¹ÝÀ» µÎ°í ÀÖ´Ù.
Glutathione-Superflow ResinÀº FPLC Àû¿ë¿¡ ÀûÇÕÇÏ´Ù. ÀÌ°ÍÀº ´õ ³ôÀº À¯¼Ó°ú 15 ml/cm2/min Á¤µµ·Î ³ôÀº À¯¼ÓÀ» °®´Â back pressureÀ» °ßµô ¼ö ÀÖ´Ù.
´õ Å« Æí¸®ÇÔÀ» À§ÇØ, GST Purification Kit´Â 5 ¹øÀÇ batch/gravity flow Á¤Á¦¸¦ À§ÇÑ ÃæºÐÇÑ stock buffer¿Í prepacked Glutathione-Superflow ColumnÀ» Á¦°øÇÑ´Ù. ÀÌ kit¸¦ »ç¿ëÇϸé column ´ç ÃÖ´ë 10 mgÀÇ GST-tagged proteinÀ» Á¤Á¦ÇÒ ¼ö ÀÖ´Ù.
Figure 1. GST-tagged protein purification from whole cell extract.
Whole cell extracts containing GST-DHFR (
Panel A
) and GST alone (
Panel B
) were loaded, washed, and eluted from glutathione resin columns. The resulting purification fractions were analyzed by SDS-PAGE (upper panels) and Western blot (lower panels) with an anti-GST IgG. WCE = whole cell extract. FT = flowthrough. ELU = eluate.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
Keyword :
´Ù¾çÇÑ tag ´Ü¹éÁú °ü·Ã
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾·ùÀÇ tag¸¦ ÀÌ¿ëÇÑ ´Ü¹éÁú »ý»ê ¹× Á¤Á¦
Excellose Spin Kit His-tagged Protein Purification
FLAG tag ´Ü¹éÁú »ý»ê
GST-tag À¶ÇÕ ´Ü¹éÁú Á¤Á¦ Resin