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´Ü¹éÁú ¹ßÇö ¡¤ Á¤Á¦
>
°ïÃæ ¼¼Æ÷ ´Ü¹éÁú ¹ßÇö (Baculovirus)
> FoldHelper Helper virus
ºÐÀÚ ChaperoneÀ» ¹ßÇöÇÏ´Â Recombinant Baculovirus
FoldHelper Helper virus
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(ºÎ°¡¼¼º°µµ)
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AB Vector
AV400
FoldHelper-ER3
2 ml, 5¡¿107 pfu/ml
°¡°Ý¹®ÀÇ
AB Vector
AV401
FoldHelper-104
1ml, 108 pfu/ml
°¡°Ý¹®ÀÇ
AB Vector
AV402
FoldHelper-104P
1ml, 108 pfu/ml
°¡°Ý¹®ÀÇ
AB Vector
AV403
FoldHelper-57P
1ml, 108 pfu/ml
°¡°Ý¹®ÀÇ
Product index
Overview
´Ü¹éÁú ¹ßÇö½Ã½ºÅÛ - »ó..
[°³¿ä] Â÷¼¼´ë ´Ü¹éÁú ..
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
[¼±Åð¡À̵å] Protein E..
His-tag À¶ÇÕ ´Ü¹éÁú Á¤Á¦
[¼±Åð¡À̵å] His-tag ..
Capturem¢â His-Tagged ..
TALON (Cobalt) resin ..
TALON Magnetic Beads
TALON (Cobalt) Superfl..
xTractor Buffer Kit
His60 (Nickel) Superfl..
His60 Ni Magnetic Bead..
HRV 3C Protease
Poly Histidine tag Ç×..
TALON (Cobalt) CellThr..
TALONSpin Column
´Ü¹éÁú ÃßÃâ ¡¤ Á¤Á¦°ü·Ã
Calpastatin
Cryonase¢â Cold-active..
Glutathione Excellose ..
IDA Excellose (polyhis..
MBP Excellose (MBP fus..
MiniExcellose
ProteoGuard EDTA-Free ..
Streptavidin-based Enr..
Ç×ü Á¤Á¦
Ç×ü Á¤Á¦ Resin
Capturem¢â IP £¦ Co-IP..
°ïÃæ ¼¼Æ÷ ´Ü¹éÁú ¹ßÇö (Bac..
[Overview] Insect Expr..
BacPAK¢â Baculovirus G..
BacPAK¢â p35 ELISA Kit..
BacPAK Baculovirus ¹ß..
Baculovirus control vi..
Baculovirus DNA packag..
Baculovirus Titer Kit
flashBAC¢â Baculovirus..
FoldHelper Helper viru..
ProEasy Baculovirus Ve..
ProFectin
ProFold Baculovirus Ve..
qPCR Titer ÃøÁ¤ Kit
Transfer Vectors
Æ÷À¯·ù ¼¼Æ÷ ´Ü¹éÁú ¹ßÇö
[Overview] Mammalian E..
CHOgro¢ç Expression Sy..
CHOgro¢ç High Yield Ex..
Corystein¢â£¨Purothion..
Human Cell-Free Protei..
pHEK293 Ultra Expressi..
´ëÀå±Õ ´Ü¹éÁú ¹ßÇö
[Overview] Bacterial E..
´ëÀå±Õ ´Ü¹éÁú ¹ßÇö £¦ ..
Anti-ProS2
Anti-TF(Trigger Factor..
Chaperone Competent Ce..
Chaperone Plasmid Set
HAT ´Ü¹éÁú ¹ßÇö£¦ Á¤Á¦..
pCold¢â GST DNA
pCold¢â ProS2 DNA
pCold¢â TF DNA
pCold¢â Vector ½Ã¸®Áî
Single Protein Product..
TaKaRa Competent Cell ..
Brevibacillus£¯Bacillus ¹ß..
B. subtilis Secretory ..
´Ù¾çÇÑ tag ´Ü¹éÁú °ü·Ã
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
Excellose Spin Kit His..
FLAG tag ´Ü¹éÁú »ý»ê
GST-tag À¶ÇÕ ´Ü¹éÁú Á¤..
´ç£¯ÀλêÈ ´Ü¹éÁú °ü·Ã
Glycoprotein ³óÃà¿ë Re..
Magnetic bead - Phosph..
Phosphoprotein enrichm..
´Ü¹éÁú ¿°»ö ½Ã¾à
TaKaRa CBB Protein Saf..
SYPRO Protein Gel Stai..
SYPRO Ruby Protein Sta..
´Ü¹éÁú °ËÃâ, Á¤·®, Çؼ®
HRP Substrate ½Ã¸®Áî
HRP-Juice
ProSieve¢â EX Running ..
TaKaRa BCA Protein Ass..
TaKaRa Bradford Protei..
Tris-Glycine Powder
Western BLoT Blocking/..
Western BLoT Immuno Bo..
Western BLoT Immuno Bo..
Western BLoT Rapid Det..
Protease (¾Æ¹Ì³ë»ê ¼¿ ÇØ..
Arginylendopeptidase (..
Pfu Aminopeptidase I
Pfu Methionine Aminope..
Pfu N-acetyl Deblockin..
Pfu Protease S
Pfu Pyroglutamate Amin..
Á¦Ç°¼³¸í
FoldHelper virus ½Ã¸®Áî´Â ƯÁ¤ ºÐÀÚ chaperoneÀ» ¹ßÇöÇÏ´Â AcNPV À¯·¡ ÀçÁ¶ÇÕ Baculovirus stockÀÌ´Ù. Baculovirus (AcNPV)´Â °ïÃæ ¼¼Æ÷ ¹ßÇö°è¸¦ ÀÌ¿ëÇÏ¿© ¸ñÀû ´Ü¹éÁúÀ» ¼¼Æ÷³»¿¡¼ °ú¹ßÇö (over-expression)Çϱâ À§ÇÑ ½Ã½ºÅÛÀ¸·Î °£È¤ inclusion body¸¦ Çü¼ºÇÏ¿© ±â´ëÇÏ´Â ¹ßÇö·®À» ¾òÀ» ¼ö ¾ø´Â °æ¿ì°¡ ÀÖ´Ù.
ÀÌ·¯ÇÑ °æ¿ì FoldHelper virus¸¦ ÀÌ¿ëÇÏ¿© ÀûÀýÇÑ chaperoneÀ» ÇÔ²² ¹ßÇö½ÃŲ´Ù. ¸ñÀû´Ü¹éÁú°ú ÇÔ²² FoldHelper virus¸¦ co-infection ½ÃÅ°´Â °ÍÀ¸·Î °£ÆíÇÏ°Ô ÀÌ¿ëÇÒ ¼ö ÀÖ´Ù.
º¸Á¸ 4¡É
Ư¡
GFP marker: virus Áõ½ÄÀ» ¸ð´ÏÅ͸µ
calreticulin: ER¿¡¼ÀÇ ´ç´Ü¹éÁú folding¿¡ °ü¿©
PDI: Cycteine-rich proteinÀÇ folding¿¡ °ü¿©
Peptidyl-prolyl cis-trans isomerases (PPIs) : proline-rich protein¿¡ Á¸ÀçÇÏ´Â imide bondÀÇ À̼ºÁúÃ¼È ÃËÁø
FoldHelper¿Í ¹ßÇöÇÏ´Â molecular chaperone, reporter ´Ü¹éÁúÀÇ Á¾·ù¿Í ¹ßÇö ·¹º§
Reporter ¶Ç´Â molecular chaperoneÀÇ Á¾·ù
GFP
¥â-gal
Hsp104
ERp57
Calreticulin
PDI
CyclophilinB
¿ªÇÒ
Áõ½Ä ¸ð´ÏÅ͸µ, ¿ª°¡ÃøÁ¤¿¡ ÀÌ¿ë
Plaque assay¿¡ ÀÌ¿ë
¼¼Æ÷ÁúÀÇ inclusion bodyÀÇ Àç»ý
ER³»°À¸·ÎÀÇ disulfide bond
ER³»°À¸·ÎÀÇ ´ç´Ü¹éÁúÀÇ folding Çü¼º
ER³»°À¸·ÎÀÇ disulfide bond Çü¼º
PPIÈ°¼º¿¡ ÀÇÇÑ imide bondÀÇ À̼ºÁúüÈ
Á¦Ç°¸í
TaKaRa Code
´Ü¹éÁú ¹ßÇö ·¹º§
FoldHelper-ER3
AV400
++
++
++
+
++
FoldHelper-104
AV401
++
FoldHelper-104P
AV402
++
++++
FoldHelper-57P
AV403
+++
++++
Keyword :
°ïÃæ ¼¼Æ÷ ´Ü¹éÁú ¹ßÇö (Baculovirus)
[Overview] Insect Expression System
10ºÐ ¿Ï·á - baculovirus ½Å¼Ó¿ª°¡ ÃøÁ¤
BacPAK¢â Baculovirus GoStix¢â Plus
°£ÆíÇØÁø baculovirus ¿ª°¡ ÃøÁ¤ Å°Æ®
BacPAK¢â p35 ELISA Kit (Single Wash)
BacPAK Baculovirus ¹ßÇö ½Ã½ºÅÛ
Positive / negative control ¿ë baculovirus
Baculovirus control virus / transfer vector
Baculovirus DNA & transfer vector set
Baculovirus DNA package kit
¸é¿ªÇÐÀû ÃøÁ¤¹ýÀ¸·Î 48 ½Ã°£À¸·Î Baculovirus ..
Baculovirus Titer Kit
°ïÃæ, Æ÷À¯·ù ¼¼Æ÷¿ë ÀçÁ¶ÇÕ ´Ü¹éÁú °í¹ßÇö ½Ã..
flashBAC¢â Baculovirus Expression System
ºÐÀÚ ChaperoneÀ» ¹ßÇöÇÏ´Â Recombinant Baculo..
FoldHelper Helper virus
Plaque Assay ¼øÈ°¡ ºÒÇÊ¿äÇÑ È¿À²ÀûÀÎ Recomb..
ProEasy Baculovirus Vectors
Sf9, Sf21 cell¿¡ ÃÖÀûÈµÈ Transfection reage..
ProFectin
Chaperone Æ÷ÇÔ - ´Ü¹éÁú °¡¿ëÈ È¿À² Çâ»ó
ProFold Baculovirus Vectors
Baculovirus ¿ª°¡£¨Genomic DNA copy ¼ö£©¸¦ ..
qPCR Titer ÃøÁ¤ Kit
¸ñÀû¿¡ µû¶ó ¼±ÅÃÇÏ¿© Baculovirus Á¦ÀÛ
Transfer Vectors