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Home > ÀüÁ¦Ç°º¸±â > PCR > High fidelity PCR: PrimeSTAR > GXL Fast Premix, dye plus
PrimeSTAR¢ç GXL Fast Premix, dye plus

GXL Fast Premix, dye plus

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
R052A
PrimeSTAR¢ç GXL Premix Fast, Dye plus
°ü·ÃÇмú ±¸¸ÅÇϱ⠻ùÇýÅû 
200ȸ (50 §¡ ±âÁØ)
360,000¿ø  R052A_DS.v2108Da.pdf
Takara
R052B
PrimeSTAR¢ç GXL Premix Fast, Dye plus
°ü·ÃÇмú ±¸¸ÅÇϱâ
800ȸ (R052A x 4)
1,296,000¿ø 
Takara
R053A
PrimeSTAR¢ç GXL Premix Fast
°ü·ÃÇмú ±¸¸ÅÇϱâ
200 ȸ
360,000¿ø  R053S_R053A_DS.v2312Da.pdf
Takara
R053B
PrimeSTAR¢ç GXL Premix Fast
°ü·ÃÇмú ±¸¸ÅÇϱâ
800 ȸ (R053Ax4)
1,296,000¿ø 

  • Á¤È®µµ ³ôÀº PCRÀ» ºü¸£°Ô!
    : extension time - 1~10 kb´Â 5ÃÊ/kb, 10 kbÀÌ»óÀº 10ÃÊ/kb ¹ÝÀÀ °¡´É
  • »ç¿ëÀÌ Æí¸®ÇÑ 2X premix ŸÀÔ
  • ³ôÀº Á¤È®µµ »Ó¸¸ ¾Æ´Ï¶ó GC rich / AT rich Ÿ°Ùµµ È¿À²ÀûÀ¸·Î ÁõÆø
Á¦Ç°¼³¸í
PrimeSTAR¢ç GXL Premix Fast´Â ´ÙÄ«¶ó¹ÙÀÌ¿À°¡ µ¶ÀÚÀûÀ¸·Î °³¹ßÇÑ high fidelity PCR È¿¼ÒÀÎ PrimeSTAR¢ç GXL DNA Polymerase¿Í PCR¿¡ ÇÊ¿äÇÑ reaction buffer, dNTP mixture°¡ ÃÖÀûÀÇ Á¶°ÇÀ¸·Î È¥ÇյǾî ÀÖ´Â 2X premixÀÌ´Ù.
¶ÇÇÑ PrimeSTAR¢ç GXL Premix Fast, Dye plus¿¡´Â Àü±â¿µµ¿¿¡ ÇÊ¿äÇÑ loading dye (ÆĶõ»ö°ú ³ë¶õ»ö)*µµ Æ÷ÇԵǾî ÀÖ¾î PCR ¹ÝÀÀ ÈÄ ¹Ù·Î Àü±â¿µµ¿ gel loadingÀÌ °¡´ÉÇÏ´Ù.
º» Á¦Ç°Àº ³ôÀº Á¤È®¼ºÀ» À¯ÁöÇϸ鼭 ªÀº ½Ã°£ ³»¿¡ ÁõÆøÀÌ °¡´ÉÇÏ°í, ÁõÆøÀÌ ¾î·Á¿ü´ø GC-rich template¿¡ ´ëÇؼ­µµ ¹öÆÛÀÇ º¯°æÀ̳ª Ưº°ÇÑ ¹ÝÀÀ Á¶°ÇÀÇ ¼³Á¤ ¾øÀÌ, ³ôÀº ¼º°øÀ²·Î ÁõÆøÇÒ ¼ö ÀÖ´Ù.
¶ÇÇÑ, º» È¿¼Ò´Â »ó¿Â¿¡¼­ DNA Polymerase È°¼º ¹× 3¡¯ ¡æ 5¡¯ exonuclease È°¼ºÀ» ¾ïÁ¦ÇÏ´Â ´ÜÀÏ Å¬·Ð Ç×ü¸¦ ÀÌ¿ëÇÑ hot start versionÀÇ PCR È¿¼ÒÀÌ´Ù.
* Àü±â¿µµ¿À» À§ÇÑ loading dye¸¸ Æ÷ÇÔ, EtBr, GelStar¢ç Nucleic Acid Stain µîÀ» ÀÌ¿ëÇÑ gel ¿°»öÀº º°µµ·Î ÁøÇà

[Note]
º» Á¦Ç°Àº In-Fusion¢ç Cloning system°ú ÇÔ²² »ç¿ëÇϸé, PCR ´ÜÆíÀ» ´Ü½Ã°£ ³»¿¡ È¿À²ÀûÀ¸·Î expression vector¿¡ cloning ÇÒ ¼ö ÀÖ´Ù.
³»¿ë (200 ȸ, 50 §¡ ¹ÝÀÀ½Ã)
PrimeSTAR¢ç GXL Premix Fast, Dye plus (Code R052A)
1. PrimeSTAR GXL Premix Fast, Dye plus (2 ¡¿)

1 §¢ x 5


PrimeSTAR¢ç GXL Premix Fast (Code R053A)
1. PrimeSTAR GXL Premix Fast (2 ¡¿)

1 §¢ x 5

º¸Á¸
-20 ¡É
PCR »ê¹°¿¡ ´ëÇÏ¿©
PrimeSTAR¢ç GXL Premix Fast, Dye plus¸¦ ÀÌ¿ëÇØ ÁõÆøÇÑ PCR »ê¹°ÀÇ ´ëºÎºÐÀº ÆòÈ° ¸»´Ü (blunt end)ÀÌ´Ù.
µû¶ó¼­ T-vector¿¡ Ŭ·Î´× ÇÏ´Â °æ¿ì, PCR »ê¹°ÀÇ 3' ¸»´Ü¿¡ dA¸¦ ºÎ°¡ÇØ¾ß Çϸç, Mighty TA-cloning Reagent Set for PrimeSTAR¢ç(Code 6019)¸¦ ÀÌ¿ëÇÏ¿© A-tailing ¹× TA cloningÀ» ÁøÇàÇÒ ¼ö ÀÖ´Ù.
PCR »ê¹°À» ±×´ë·Î (ÇÊ¿ä¿¡ µû¶ó¼­ ÀλêÈ­ÇÏ¿©) ÆòÈ° ¸»´Ü º¤ÅÍ¿¡ Ŭ·Î´×ÇÏ´Â °æ¿ì Mighty Cloning Reagent Set (Blunt End) (Code 6027)¸¦ ÀÌ¿ëÇÒ ¼ö ÀÖ´Ù.
°ü·ÃÁ¦Ç°
- A-tailing & TA cloning kit: Mighty TA-cloning Reagent Set for PrimeSTAR¢ç
- Á¤È®ÇÑ cDNA ÇÕ¼ºÀ» À§ÇÑ ¿ªÀü»ç¹ÝÀÀ kit: PrimeScript¢â II 1st strand cDNA Synthesis Kit
- Gel elution¿ë Agarose: SeaPlaque¢ç Agarose
- 5ºÐ¸¸¿¡ Àü±â¿µµ¿ ¿Ï·á! Precast gel system: FlashGel¢â System
- ÄÄÆÑÆ®ÇÑ 96 well gradient PCR ±â±â : Clontech PCR Thermal Cycler GP

Keyword :

High fidelity PCR: PrimeSTAR
>
Long fragment ÁõÆø ¹× GC-rich template ÁõÆø..
Á¤È®ÇÑ GXL DNA Polymerase
>
Á¤È®µµ ³ôÀº PrimeSTAR¢ç GXLÀÇ Premix type
GXL Premix ŸÀÔ
>
ÃÖ°í ¼öÁØÀÇ Á¤È®¼ºÀº À¯Áö, °í¼Ó PCR ¼º´É ´ë..
ÃÖ°í Á¤È®µµ-Max v2.
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ÃÖ°í ·¹º§ÀÇ Á¤È®¼º £¦ ÃÖ°í ¼ÓµµÀÇ ½ÅÀ强
ÃÖ°í Á¤È®µµ-Max DNA Polymerase
>
High Fidelity PCR È¿¼ÒÀÇ ±âÁØ
񃧯-HS DNA Polymerase
>
Premix TypeÀÇ High Fidelity PCR È¿¼Ò
HS (Premix)
>
GC-rich Template¿¡ °­ÇÑ High Fidelity PCR È¿..
HS with GC Buffer
>
PrimeSTAR¢ç GXL Fast Premix, dye plus
GXL Fast Premix, dye plus