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TaKaRa
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  • ¾÷¹«¾È³»¦¢´ëÀü/ÃæûÁö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
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Recombinant Taq DNA Polymerase

Recombinant Taq

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
R001A
TaKaRa Taq¢â
°ü·ÃÇмú ±¸¸ÅÇϱâ
250 U
107,000¿ø  R001A_DS.v1905Da.pdf
Takara
R001B
TaKaRa Taq¢â
°ü·ÃÇмú ±¸¸ÅÇϱâ
1,000 U
383,000¿ø 
Takara
R001AM
TaKaRa Taq¢â (Mg2+ free Buffer ÷ºÎ)
°ü·ÃÇмú ±¸¸ÅÇϱâ
250 U
107,000¿ø  R001AM_DS.v1905Da.pdf
Takara
R001BM
TaKaRa Taq¢â (Mg2+ free Buffer ÷ºÎ)
°ü·ÃÇмú ±¸¸ÅÇϱâ
1,000 U (R001AM x 4)
380,000¿ø  R001AM_DS.v1905Da.pdf


Á¦Ç° ¼³¸í
º» Á¦Ç°Àº Thermus aquaticus DNA polymerase À¯ÀüÀÚ¸¦ cloning ÈÄ ÇüÁúÀüȯÇÑ ´ëÀå±ÕÀ¸·ÎºÎÅÍ Á¤Á¦µÈ 94 kDaÀÇ recombinant Taq polymerase·Î õ¿¬ÀÇ Taq DNA polymerase¿Í µ¿µîÇÑ ±â´ÉÀ» °®°í ÀÖ´Ù.
³»¿ë (250 U)
TaKaRa Taq (Code R001A)
1. TaKaRa Taq (5 U/§¡)
50 §¡
2. 10¡¿ PCR Buffer (Mg2+ plus)
1 §¢
3. dNTP Mixture (°¢ 2.5 mM)
800 §¡
TaKaRa Taq¢â(Mg2+ free Buffer) (Code R001AM)
1. TaKaRa Taq (5 U/§¡)
50 §¡
2. 10¡¿ PCR Buffer (Mg2£« free)
1 §¢
3. MgCl2 (25 mM)
1 §¢
4. dNTP Mixture (°¢ 2.5 mM)
800 §¡
º¸Á¸ -20¡É
Çü»ó

20 mM

Tris-HCl (pH8.0)

100 mM

KCl

0.1 mM

EDTA

1 mM

DTT

0.5%

Tween 20

0.5%

NP-40

50%

Glycerol

÷ºÎ 10 ¡¿ PCR Buffer Á¶¼º

10¡¿ PCR Buffer (Mg2+ plus)

100 mM

Tris-HCl (pH8.9)

500 mM

KCl

15 mM

MgCl2

10¡¿ PCR Buffer (Mg2+ free)

100 mM

Tris-HCl (pH8.9)

500 mM

KCl

÷ºÎ dNTP Mixture Á¶¼º*

³óµµ

°¢ 2.5 mM

Çü»ó

¼ö¿ë¾× (³ªÆ®·ý¿°), pH7~9

¼øµµ

°¢ 98% ÀÌ»ó

*dATP, dCTP, dGTP, dTTPµî ¸ô È¥ÇÕ¹°·Î Èñ¼®ÇÏÁö ¾Ê°í ±×´ë·Î PCR¹ÝÀÀ¿¡ ÀÌ¿ëÇÒ ¼ö ÀÖ´Ù.
PCR »ê¹°¿¡ ´ëÇÏ¿©
TaKaRa Taq¢âÀ» ÀÌ¿ëÇÏ¿© ÁõÆøÇÑ PCR »ê¹°ÀÇ ´ëºÎºÐÀº 3¡¯ ¸»´Ü¿¡ A°¡ 1¿°±â ºÎ°¡µÇ¾î ÀÖ´Ù. µû¶ó¼­ ÀÌ PCR »ê¹°À» ±×´ë·Î T-vector pMD20 (Code 3270) ¶Ç´Â T-vector pMD19 (Simple) (Code 3271)¿¡ cloningÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ ¸»´Ü ÆòÈ°È­ ¹× ÀλêÈ­ ÈÄ, ÆòÈ° ¸»´Ü vector¿¡ cloningÇÒ ¼öµµ ÀÖ´Ù.
°ü·ÃÁ¦Ç°
- ºñƯÀÌÀûÀÎ ÁõÆøÀ» ¹æÁö: TaKaRa Taq¢â Hot Start Version
- 2X PCR master mix: Premix Taq¢â (TaKaRa Taq¢â Version 2.0) (500 ¥ìl¡¿6 tube)
- ´Ù¾çÇÑ »ùÇÃÀÇ DNA Á¤Á¦¿¡ ÃÖÀû: TaKaRa MiniBEST Universal Genomic DNA Extraction Kit
- 1kb ÀÌÇÏÀÇ PCR »ê¹°¿¡ ÃÖÀûÀÇ agarose: NuSieve¢â 3:1 Agarose
- ¿Ã¶ó¿îµå Agarose: SeaKem¢ç LE Agarose
- 5ºÐ¸¸¿¡ Àü±â¿µµ¿ ¿Ï·á! Precast gel system: FlashGel¢â System
- ÄÄÆÑÆ®ÇÑ 96 well gradient PCR ±â±â : Clontech PCR Thermal Cycler GP

Keyword : r001,rtaq,r taq

Standard PCR: Ex, LA, rTaq
>
1st choice PCR È¿¼Ò
TaKaRa Ex Premier¢â DNA Polymerase
>
Hot start PCR·Î ºñƯÀÌÀûÀÎ ÁõÆøÀ» ¹æÁö
Ex Taq Hot Start Version
>
°íÈ¿À², °í°¨µµ – PCR È¿¼ÒÀÇ standard
TaKaRa Ex Taq
>
Hot start PCR·Î ºñƯÀÌÀûÀÎ ÁõÆøÀ» ¹æÁö
LA Taq Hot Start Version
>
±ä ´ÜÆíÀÇ DNA / GC rich ¼­¿­ ÁõÆø
LA Taq
>
Hot start PCR·Î ºñƯÀÌÀûÀÎ ÁõÆøÀ» ¹æÁö
Taq Hot Start Version
>
Recombinant Taq DNA Polymerase
Recombinant Taq
>
½Ç¿Â º¸°ü °¡´ÉÇÑ master mix
µ¿°á°ÇÁ¶ ºÐÁÖÇüÅÂ-Taq HS Premix
>
Fast PCR¿ë hot start È¿¼Ò
SpeedSTAR HS È¿¼Ò
>
°í¼Ó PCRÀ» À§ÇÑ
Fast PCR¿ë Z-Taq
>
Hot Start PCR ¿ë Anti-Taq Antibody
Taq Antibody
>
»ç¿ëÀÌ Æí¸®ÇÑ
Premix ½Ã¾à (Ex, LA Taq)
>
2¡¿ Premix PCR Solution
Premix ½Ã¾à (Hot Start Version)