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PCR
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High fidelity PCR: PrimeSTAR
> GXL Fast Premix, dye plus
PrimeSTAR¢ç GXL Fast Premix, dye plus
GXL Fast Premix, dye plus
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Takara
R052A
PrimeSTAR¢ç GXL Premix Fast, Dye plus
200ȸ (50 §¡ ±âÁØ)
360,000¿ø
Takara
R052B
PrimeSTAR¢ç GXL Premix Fast, Dye plus
800ȸ (R052A x 4)
1,296,000¿ø
Takara
R053A
PrimeSTAR¢ç GXL Premix Fast
200 ȸ
360,000¿ø
Takara
R053B
PrimeSTAR¢ç GXL Premix Fast
800 ȸ (R053Ax4)
1,296,000¿ø
Product index
Overview
PCR FAQs - ÃßõÇÏ´Â PC..
PCR FAQs - Optimizing ..
High Fidelity È¿¼Ò-Pri..
PCR¡¢LA PCR¢â¡¢Hot Sta..
[Cancer Research] HLA ..
½ÇÇ躰 PCR È¿¼Ò ¼±ÅÃÇÏ..
High fidelity PCR: PrimeST..
Á¤È®ÇÑ GXL DNA Polymer..
GXL Premix ŸÀÔ
ÃÖ°í Á¤È®µµ-Max v2.
ÃÖ°í Á¤È®µµ-Max DNA Po..
񃧯-HS DNA Polymerase
HS (Premix)
HS with GC Buffer
Pyrobest DNA Polymeras..
GXL Fast Premix, dye p..
Standard PCR: Ex, LA, rTaq..
TaKaRa Ex Premier¢â DN..
Ex Taq Hot Start Versi..
TaKaRa Ex Taq
LA Taq Hot Start Versi..
LA Taq
Taq Hot Start Version
Recombinant Taq
µ¿°á°ÇÁ¶ ºÐÁÖÇüÅÂ-Taq ..
SpeedSTAR HS È¿¼Ò
Fast PCR¿ë Z-Taq
Taq Antibody
Premix ½Ã¾à (Ex, LA Ta..
Premix ½Ã¾à (Hot Start..
¾÷±×·¹À̵å Taq HS Prem..
Direct PCR: MightyAmp
°ÇÑ ÁõÆø´É·Â- Ver.3
Genotyping¿ë ÃßÃâ½Ã¾à..
񃧯-Ver.2
Dye°¡ Æ÷ÇÔµÈ premix
Colony PCR¿ë premix
Hot Start Version prem..
Fast PCR¿ë premix
PCR¿ë Premix ½Ã¸®Áî
Ư¼ö PCR (Epigenetics, UNG..
EpiTaq HS (for bisulfi..
Multiplex PCR Kit Ver...
¼÷ÁÖÀ¯·¡ DNA ¾ïÁ¦-Taq ..
BcaBEST¢ç DNA Polymera..
TaKaRa Taq¢â HS PCR Ki..
Uracil DNA Glycosylase..
PCR Enzyme (Clontech)
Clontech PCR Enzyme ¼±..
[°³¿ä] So Easy Product
[Cancer Research] Canc..
Advantage 2 Polymerase..
Advantage GC 2 Polymer..
Advantage Genomics LA ..
Advantage HD Polymeras..
Advantage HF 2 PCR Kit
CloneAmp HiFi PCR Prem..
High Fidelity PCR EcoD..
High Yield PCR EcoDry ..
SeqAmp¢â DNA Polymeras..
SuperPlex¢â Premix
Terra PCR Direct Polym..
TITANIUM¢ç Taq DNA Pol..
DNA PCR °ü·Ã Á¦Ç°
Diversify PCR Random M..
LA PCR¢â ¿ë genome DNA..
TaKaRa LA PCR¢â Kit Ve..
TaKaRa PCR Amplificati..
RT-PCR °ü·Ã Á¦Ç°
PrimeScript¢â II High ..
RT-PCR kit, ²Ä²ÄÈ÷ µû..
BcaBEST¢ç RNA PCR Kit ..
PrimeScript¢â High Fid..
PrimeScript¢â One Step..
PrimeScript¢â RT-PCR K..
SMARTer¢ç RACE 5'/3' K..
TaKaRa One Step RNA PC..
TaKaRa RNA LA PCR¢â Ki..
TaKaRa RNA PCR¢â Kit (..
Mutagenesis
Diversify PCR Random M..
PCR Subtraction °ü·Ã Á¦Ç°
Clontech PCR-Select Ba..
Clontech PCR-Select cD..
Clontech PCR-Select Di..
PCR ÀåÄ¡
PCR °ü·Ã ¼Ò¸ðÇ°
TaKaRa PCR Thermal Cyc..
PCR Accessory Products
Advantage Ultrapure PC..
TaqStart Antibody
Whole Genome SNP Detection
TITANIUM DNA AMPLIFICA..
º´¿øü¤ýÀ¯ÀüÀÚ °ËÃâ (PCR)
¸Åµ¶¿ø¼º ´ëÀå±Õ °ËÃâ¿ë..
Àå°ü ÃâÇ÷¼º ´ëÀå±Õ ¿ë ..
Àå¿° Vibrio °ËÃâ¿ë Pri..
ÄÝ·¹¶ó µ¶¼Ò °ËÃâ¿ë Pri..
Ư¼ö ¼¼±Õ °ËÃâ¿ë Posit..
Ȳ»öÆ÷µµ»ó±¸±Õ °ËÃâ¿ë ..
Bacterial 16S rDNA PCR..
Bacterial 16S rDNA PCR..
Botulinus±Õ °ËÃâ¿ë Pri..
Fungal rDNA (ITS1) PCR..
O-157(º£·Î µ¶¼Ò À¯ÀüÀÚ..
Salmonella±Õ °ËÃâ¿ë Pr..
Shigella ¹× Àå°üħÀÔ¼º..
TaKaRa źÀú±Õ PCR Dete..
TaKaRa PCR Mycoplasma ..
Welchii±Õ °ËÃâ¿ë Prime..
Á¤È®µµ ³ôÀº PCRÀ» ºü¸£°Ô!
: extension time - 1~10 kb´Â 5ÃÊ/kb, 10 kbÀÌ»óÀº 10ÃÊ/kb ¹ÝÀÀ °¡´É
»ç¿ëÀÌ Æí¸®ÇÑ 2X premix ŸÀÔ
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PrimeSTAR
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GXL Premix Fast´Â ´ÙÄ«¶ó¹ÙÀÌ¿À°¡ µ¶ÀÚÀûÀ¸·Î °³¹ßÇÑ high fidelity PCR È¿¼ÒÀÎ PrimeSTAR
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GXL DNA Polymerase¿Í PCR¿¡ ÇÊ¿äÇÑ reaction buffer, dNTP mixture°¡ ÃÖÀûÀÇ Á¶°ÇÀ¸·Î È¥ÇյǾî ÀÖ´Â 2X premixÀÌ´Ù.
¶ÇÇÑ PrimeSTAR
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GXL Premix Fast, Dye plus¿¡´Â Àü±â¿µµ¿¿¡ ÇÊ¿äÇÑ loading dye (ÆĶõ»ö°ú ³ë¶õ»ö)*µµ Æ÷ÇԵǾî ÀÖ¾î PCR ¹ÝÀÀ ÈÄ ¹Ù·Î Àü±â¿µµ¿ gel loadingÀÌ °¡´ÉÇÏ´Ù.
º» Á¦Ç°Àº ³ôÀº Á¤È®¼ºÀ» À¯ÁöÇÏ¸é¼ ÂªÀº ½Ã°£ ³»¿¡ ÁõÆøÀÌ °¡´ÉÇÏ°í, ÁõÆøÀÌ ¾î·Á¿ü´ø GC-rich template¿¡ ´ëÇؼµµ ¹öÆÛÀÇ º¯°æÀ̳ª Ưº°ÇÑ ¹ÝÀÀ Á¶°ÇÀÇ ¼³Á¤ ¾øÀÌ, ³ôÀº ¼º°øÀ²·Î ÁõÆøÇÒ ¼ö ÀÖ´Ù.
¶ÇÇÑ, º» È¿¼Ò´Â »ó¿Â¿¡¼ DNA Polymerase È°¼º ¹× 3¡¯ ¡æ 5¡¯ exonuclease È°¼ºÀ» ¾ïÁ¦ÇÏ´Â ´ÜÀÏ Å¬·Ð Ç×ü¸¦ ÀÌ¿ëÇÑ hot start versionÀÇ PCR È¿¼ÒÀÌ´Ù.
* Àü±â¿µµ¿À» À§ÇÑ loading dye¸¸ Æ÷ÇÔ, EtBr,
GelStar
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Nucleic Acid Stain
µîÀ» ÀÌ¿ëÇÑ gel ¿°»öÀº º°µµ·Î ÁøÇà
[Note]
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In-Fusion
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Cloning system
°ú ÇÔ²² »ç¿ëÇϸé, PCR ´ÜÆíÀ» ´Ü½Ã°£ ³»¿¡ È¿À²ÀûÀ¸·Î expression vector¿¡ cloning ÇÒ ¼ö ÀÖ´Ù.
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PrimeSTAR
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GXL Premix Fast, Dye plus (Code R052A)
1. PrimeSTAR GXL Premix Fast, Dye plus (2 ¡¿)
1 §¢ x 5
PrimeSTAR
¢ç
GXL Premix Fast (Code R053A)
1. PrimeSTAR GXL Premix Fast (2 ¡¿)
1 §¢ x 5
º¸Á¸
-20 ¡É
PCR »ê¹°¿¡ ´ëÇÏ¿©
PrimeSTAR
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GXL Premix Fast, Dye plus¸¦ ÀÌ¿ëÇØ ÁõÆøÇÑ PCR »ê¹°ÀÇ ´ëºÎºÐÀº ÆòÈ° ¸»´Ü (blunt end)ÀÌ´Ù.
µû¶ó¼ T-vector¿¡ Ŭ·Î´× ÇÏ´Â °æ¿ì, PCR »ê¹°ÀÇ 3' ¸»´Ü¿¡ dA¸¦ ºÎ°¡ÇØ¾ß Çϸç,
Mighty TA-cloning Reagent Set for PrimeSTAR
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(Code 6019
)¸¦ ÀÌ¿ëÇÏ¿© A-tailing ¹× TA cloningÀ» ÁøÇàÇÒ ¼ö ÀÖ´Ù.
PCR »ê¹°À» ±×´ë·Î (ÇÊ¿ä¿¡ µû¶ó¼ ÀλêÈÇÏ¿©) ÆòÈ° ¸»´Ü º¤ÅÍ¿¡ Ŭ·Î´×ÇÏ´Â °æ¿ì
Mighty Cloning Reagent Set (Blunt End) (Code 6027)
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°ü·ÃÁ¦Ç°
- A-tailing & TA cloning kit:
Mighty TA-cloning Reagent Set for PrimeSTAR
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- Á¤È®ÇÑ cDNA ÇÕ¼ºÀ» À§ÇÑ ¿ªÀü»ç¹ÝÀÀ kit:
PrimeScript¢â II 1st strand cDNA Synthesis Kit
- Gel elution¿ë Agarose:
SeaPlaque
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Agarose
- 5ºÐ¸¸¿¡ Àü±â¿µµ¿ ¿Ï·á! Precast gel system:
FlashGel¢â System
- ÄÄÆÑÆ®ÇÑ 96 well gradient PCR ±â±â :
Clontech PCR Thermal Cycler GP
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Keyword :
High fidelity PCR: PrimeSTAR
Long fragment ÁõÆø ¹× GC-rich template ÁõÆø..
Á¤È®ÇÑ GXL DNA Polymerase
Á¤È®µµ ³ôÀº PrimeSTAR¢ç GXLÀÇ Premix type
GXL Premix ŸÀÔ
ÃÖ°í ¼öÁØÀÇ Á¤È®¼ºÀº À¯Áö, °í¼Ó PCR ¼º´É ´ë..
ÃÖ°í Á¤È®µµ-Max v2.
ÃÖ°í ·¹º§ÀÇ Á¤È®¼º £¦ ÃÖ°í ¼ÓµµÀÇ ½ÅÀ强
ÃÖ°í Á¤È®µµ-Max DNA Polymerase
High Fidelity PCR È¿¼ÒÀÇ ±âÁØ
񃧯-HS DNA Polymerase
Premix TypeÀÇ High Fidelity PCR È¿¼Ò
HS (Premix)
GC-rich Template¿¡ °ÇÑ High Fidelity PCR È¿..
HS with GC Buffer
Pyrobest DNA Polymerase
PrimeSTAR¢ç GXL Fast Premix, dye plus
GXL Fast Premix, dye plus