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Home > ÀüÁ¦Ç°º¸±â > PCR > Ư¼ö PCR (Epigenetics, UNG µî) > Uracil DNA Glycosylase (UNG), heat-labile
¾ÈÁ¤µÈ PCR °á°ú È®ÀÎ, ¿­ ¹Î°¨¼ºÀÌ ³ôÀº

Uracil DNA Glycosylase (UNG), heat-labile

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
2820
Uracil DNA Glycosylase (UNG), heat-labile
°ü·ÃÇмú ±¸¸ÅÇϱâ
200 U(200 ȸ)
259,000¿ø  2820_DS.v1901Da.pdf
Takara
4035
dU plus dNTP Mixture(12.5¡¿)
°ü·ÃÇмú ±¸¸ÅÇϱâ
800 ¥ìl
139,000¿ø  4035_DS.v1812Da.pdf

Á¦Ç° ¼³¸í
Uracil DNA Glycosylase (UNG)´Â ¿ì¶ó½ÇÀ» Æ÷ÇÔÇÏ´Â DNA¿¡¼­ deoxyribose ¿Í ¿ì¶ó½Ç (U) ¿°±â »çÀÌÀÇ N-glycosylic bond¸¦ °¡¼öºÐÇØÇÏ¿© Å»Çʸ®¹Ìµò ºÎÀ§¸¦ ¸¸µå´Â ¿ªÇÒÀ» ÇÑ´Ù. º» È¿¼Ò´Â ¿ì¶ó½ÇÀ» Æ÷ÇÔÇÑ single strand DNA¿Í double strand DNA´Â °¡¼öºÐÇØÇÏÁö¸¸, RNA¿¡´Â ÀÛ¿ëÇÏÁö ¾Ê´Â´Ù.

*TaKaRa Taq¢â HS PCR È¿¼Ò¿Í UNG ½Ã½ºÅÛÀÌ ÇÔ²² ±¸¼ºµÈ kit´Â TaKaRa Taq¢â HS PCR Kit, UNG plus (Code R013S)·Î ±¸¸ÅÇÏ½Ç ¼ö ÀÖ½À´Ï´Ù.
Çü»ó

20 mM

 

Tris-HCl, pH8.0 (at 4¡É)

100 mM

KCl

1 mM

DTT

0.1 mM

EDTA

0.5£¥(v/v)

NP-40

0.5£¥(v/v)

Tween 20

50£¥(v/v)

Glycerol

±â¿ø
E. coli strain expressing a recombinant Xiphophorus maculatus UNG mutant.
ºÒÈ°¼ºÈ­ Á¶°Ç
º» È¿¼Ò´Â 50¡É, 10ºÐ ¿­Ã³¸®¿¡ ÀÇÇØ ºñ°¡¿ªÀûÀ¸·Î ¿ÏÀüÈ÷ ºÒÈ°¼ºÈ­ µÈ´Ù.
º¸Á¸
-20¡É
°ü·ÃÁ¦Ç°
- ÇÔ²² »ç¿ëÇϸé ÁÁÀº PCR È¿¼Ò
   : TaKaRa Taq¢â Hot Start Version
   : TaKaRa Taq¢â
- UNG°¡ Æ÷ÇÔµÈ ´Ù¾çÇÑ Á¦Ç°
   : qPCR premix: Probe qPCR Mix, with UNG
   : 1 step RT-qPCR mix: One Step PrimeScript¢â III RT-qPCR Mix, with UNG
   : RNA Á¤Á¦¾øÀÌ direct RT-qPCR mix: PrimeDirect¢ç Probe RT-qPCR Mix, with UNG
- ÄÄÆÑÆ®ÇÑ 96 well gradient PCR ±â±â : Clontech PCR Thermal Cycler GP

Keyword :

Ư¼ö PCR (Epigenetics, UNG µî)
>
Bisulfite ó¸® ÈÄÀÇ DNA PCR ÁõÆø¿¡ ÃÖÀû
EpiTaq HS (for bisulfite-treated DNA)
>
ƯÀ̼º ³ôÀº high-plex PCR¿¡ ÃÖÀû
Multiplex PCR Kit Ver.2
>
Low DNA ÁõÆøÀ» À§ÇÑ
¼÷ÁÖÀ¯·¡ DNA ¾ïÁ¦-Taq HS
>
ÇÙ»êµî¿ÂÁõÆø¿ë PCR È¿¼Ò
BcaBEST¢ç DNA Polymerase ver.2.0
>
Carryover ¿À¿°À¸·Î ÀÌÇÑ false positive ¹æÁö ..
TaKaRa Taq¢â HS PCR Kit, UNG plus
>
¾ÈÁ¤µÈ PCR °á°ú È®ÀÎ, ¿­ ¹Î°¨¼ºÀÌ ³ôÀº
Uracil DNA Glycosylase (UNG), heat-labile